Team:Exeter/lab book/1gp/wk6

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ExiGEM2012 Lab Book 1GP wk6

Single Gene Plasmids and Enzyme Characterisation: 13th - 24th August 2012

Monday 13th August (9.00) – PCR amplification of WbbC from BL21(DE3), BioBrick assembly, Gel Electrophoresis and Preparation of LB agar and broth

Tuesday 14th August (9.00) – 3A assembly and Transformation

Wednesday 15th August (15.00) – Adding Cultures to Liquid Medium

Thursday 16th August (9.00) – Mini-Prepping, 3A assembly and Gel Electrophoresis