"

From 2012.igem.org

Contents 1 Protocol 1.1 Medium & Buffer recipes 1.2 CCMV Coat Protein VLP formation 1.3 Hepatitis B Coat Protein VLP formation 1.4 PoLeRo Coat Protein VLP formation 1.5 General Protocol

Protocol

Medium & Buffer recipes

• LB medium
• SOB medium
• SOC medium
• Disassembly buffer
• Dialysis buffer 10x
• Reassembly buffer
• Virus buffer
• Towbin's electrotransfer buffer 1x
• Towbin's electrotransfer buffer 10x

CCMV Coat Protein VLP formation

• Growing culture
• Dialysis of the VLPs
• Purifing the VLPs

Hepatitis B Coat Protein VLP formation

PoLeRo Coat Protein VLP formation

• RNA isolation from potato leaf material

General Protocol

• Preparation of electrocompetent cells of E. coli
• SDS-Polyacrylamide and native gel electrophoresis
• Dynamic Light Scattering user manual
• French press user manual