Team:HokkaidoU Japan/Notebook/Week 3

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• Lab Diary - Aggregation
  • Week 1 (Jul.2nd ~ Jul.8th)
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• Lab Diary - PHB
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• Protocols
  • General Protocols
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July 16th

Digestion

Results of digestion in 15th.

Digestion result image

Product:Ag43(K346007)=3120bp and 2070bp, pT7-RBS on pSB1K3=2247bp We confirmed there are some kind of restriction enzyme site in K346007 (digested with SpeI, PstI) and pT7-RBS on pSB1K3 was successfully digested with EcoRI and PstI.　Balance between d+(EcoRI) and d+(E&P:cut with EcoRI and PstI) is about 80bp.