Team:Trieste/notebook10

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Week 10

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Suicide System

We transformed also the double terminator (BBa_B0015) as Bglbrick. We amplified by PCR the BBa_B0015 miniprep with the appropriate PCR protocol. We loaded the gel with the amplified and the eluted was ligated in the pGEM vector.

The plasmid was amplified with the primers SP6 and T7 and then cut BglII/XhoI.

For the final construct -holin LL 37- :the LL 37 (BBa_K875009) was cloned downstream the T5CumateOperator (BBa_K875001), heat inactivated and dephosphorylated at the extremities.

Antibody

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Chassis

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