Team:Exeter/lab book/gibs/wk1

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ExiGEM2012 Lab Book Gibs wk1

Operon Construction: 9th - 13th July 2012

12.7.12

Chose three colonies from each plate.

Transferred aseptically into 1:1000 ampicillin:LB broth.

Incubated at 37*C, 220rpm overnight.

13.7.12

Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes.