Interactive pattern of our construction : Toggle switch option

Hover your mouse over a step number/letter to see more

Our parts :

Data about our favorite parts :

Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis Constitutive Promoter (P_veg) with the lysostaphin gene. In our plasmid collection, this part is named pBK23 in the pSB1C3 backbone (Cm^R) and pBKL28 in the shuttle vector E. coli – B. subtilis. The pBKL28 plasmid was transformed in the NM522 strain to perform tests in E. coli and in the 168 strain to perform tests in Bacillus subtilis.

Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis Constitutive Promoter (P_veg) with the dispersin B gene (dspB). In our plasmid collection, this part is named pBK33 in the Chloramphenicol backbone and pBKH41 in the shuttle vector E. coli – B. subtilis. We worked with the plasmid pBKH41 for the tests and we tried two different genetic backgrounds : the NM522 strain to make test in E. coli and the 168 strain to make test in Bacillus subtilis.

Main Page: P_lac(B. subtilis)-RBS(E. coli)-GFP BBa_K802002 : This part is used to determine if the promoter P_lac works or not and especially how it works when it is activated by different concentration of the inductor IPTG. Within this part there is P_lac from Bacillus subtilis which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from E coli is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites.

Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites.

Main Page: iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004 BBa_K802005 : This part is a custom designed polylinker which was cloned into the shuttle vectors BBa_K802004 and BBa_K802003 so that the plasmids would correspond to the iGEM format.