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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
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Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks: | Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks: | ||
| - | * +T7 promoter Agrobacterium tumefaciens bacteriophytochrome | + | * [[media:AgroT7A.pdf | +T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] |
| - | * -T7 promoter Agrobacterium tumefaciens bacteriophytochrome | + | * [[media:AgroA.pdf | -T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] |
| - | * +T7 promoter Heme oxygenase | + | * [[media:T7Heme.pdf | +T7 promoter Heme oxygenase ]] |
| - | * -T7 promoter Heme oxygenase | + | * [[media:HemeA.pdf | -T7 promoter Heme oxygenase ]] |
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties. | Click on each above to see the corresponding fragment nucleotide sequence and fragment properties. | ||
Revision as of 08:16, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
Biobrick Name Part(s) for BioBrick T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B Heme oxygenase (T7 minus) Plasmid Hemo_A Hemo_B T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E Agro (T7 minus) Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E
