Team:Wisconsin-Madison

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  Project Description Overviews</h1></center>
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<p align="center">ENGINEERING E. COLI TO PRODUCE LIMONENE</p>
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<p align="center">Limonene is a colorless, liquid hydrocarbon that smells like lemon. It is found in the oils of citrus fruits and is primarily used as a cleaning agent, solvent, and flavorful food additive. Limonene also possesses chemical properties that make it an ideal jet fuel due to it's low freezing point and high combustibility. E. coli already has part of the metabolic pathway required to produce limonene, however we will be inserting genes from S. cerevisiae to create the mevalonate pathway in the cell. We will also codon optimize the Limonene Synthase (LimS1) gene which is the final step in converting GPP to limonene. Gas chromatography/mass spectrometry will be used to determine how much limonene the E. coli is producing.
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      <a id="crest" href="http://www.wisc.edu"><img src="images/crest.png" alt="UW–Madison crest." width="70" height="106" /></a>
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      <p align="center">SURFACE DISPLAY AND FLOCCULATION</p>
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      <p align="center">Engineering an easy cut and paste method to display proteins</p>
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      <p align="center">Many previous iGEM teams have had success displaying a number of different enzymes and proteins on the surface of a cell. However, many of the methods used were specific to a lab at their respective universities. We wanted to create a common “display vector” that all teams can use to display any part they would like. This vector also allows for the use of standard digestion and ligation protocols, and not newer cloning methods like Gibson or PIPE. We also wanted to characterize a surface displayed Beta-Glucosidase part which would allow the degradation of cellobiose, which has large implications for the alternative fuel industry.</p>
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      <p align="center">Flocculation</p>
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      <p align="center">Flocculation is the process of individual cells, suspended in solution, coming together to form larger clumps, subsequently settling out of solution. The practical applications of having strains of bacteria capable of doing this in response to an inducible signal are numerous. This project seeks to us the high affinity binding interaction between the LamB maltoporin, and gpJ—a truncated portion of the C-terminal of the J protein of the lambda bacteriophage. gpJ is the region of the J protein that is responsible for the binding of the lambda phage to e. coli, at its surface receptor—LamB. By surfacing displaying a fusion of gpJ and maltose binding protein (MBP) with ice nucleation protein (INP), and over-expressing LamB in response to IPTG induction, we hope to produce a form of inducible flocculation in E. Coli Cells. Furthermore, by using the sigma-S promoter, we hope to make this flocculation occur automatically at the beginning of the stationary phase of the cell cycle. This would make the system ideal for a bioreactor which produces a growth phase metabolite, such as limonene.</p>
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Revision as of 02:42, 28 August 2012

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd"> Your Department Name | University of Wisconsin–Madison

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Arcu tellus wisi lacus laoreet, placerat pede sem laoreet commodi, mauris arcu tincidunt ultricies, justo euismod lacus ipsum lacinia. Enim ac lobortis dolor, faucibus nunc arcu sagittis tincidunt, dignissimos duis odio tortor ultricies. Et aliquet justo sed porttitor, nulla volutpat dui dignissim vestibulum, enim wisi erat turpis.

Samantha Baker (left) and Jose Zepeda (center) watch scientist Lia Kent (right) working as they participate in a summer science camp hosted at the WiCell Research Institute at University Research Park. Credit: University Communications

Tempus cras praesent blandit urna, accumsan nisl parturient varius dictum, amet mauris non lorem dignissim. Urna molestie ut aenean ipsum, sodales egestas porttitor urna posuere, ut et mauris praesent adipiscing, a consequat neque imperdiet augue. Quis proin sed magna dolor, lorem nulla proin platea, tempor et turpis duis vitae. Ac quisque convallis amet sit, pede risus vulputate nam lectus.

Section header

Malesuada dictum nostra ligula, lacus eget imperdiet pede, eu eleifend tempus amet, nonummy tincidunt lacinia vitae. Harum vel vitae egestas, dictum faucibus convallis lorem, mauris at felis eget, morbi maecenas in interdum accumsan. At nec pede id, aliquet ipsum velit repudiandae.

Diam blandit dui ultrices maecenas, vel nullam amet lorem, vulputate sem sodales egestas condimentum. Etiam ipsum vel nisl, sit interdum ullamcorper nulla, praesent condimentum enim vestibulum, ligula tempus luctus mollis. Nec ut id pellentesque, netus felis diam dui lectus. Lorem leo sed suspendisse dui, sapien perspiciatis aliquam arcu at, adipiscing pede porta quam pede. Molestie imperdiet sed ut, mauris vulputate molestie mauris arcu. Turpis tincidunt purus sit, ipsum auctor orci mi mauris:

  • Lorem ipsum dolor sit amet, sagittis arcu odio dolorem, praesent magna voluptate hendrerit, ullamcorper mollis non dolor.
  • Curabitur in egestas ullamcorper dignissim, lectus arcu suspendisse diam imperdiet, porttitor lacinia blandit habitant dolor.
  • Sagittis habitasse platea elit, imperdiet cum nonummy curabitur.
  • Porta varius consectetuer pellentesque, quam etiam dui praesent.

Lorem ipsum dolor sit amet!

Arcu tellus wisi lacus laoreet, placerat pede sem laoreet commodi, mauris arcu tincidunt ultricies, justo euismod lacus ipsum lacinia. Enim ac lobortis dolor, faucibus nunc arcu sagittis tincidunt, dignissimos duis odio tortor ultricies. Et aliquet justo sed porttitor, nulla volutpat dui dignissim vestibulum, enim wisi erat turpis.

  1. Lorem ipsum dolor sit amet, sagittis arcu odio dolorem, praesent magna voluptate hendrerit, ullamcorper mollis non dolor.
  2. Curabitur in egestas ullamcorper dignissim, lectus arcu suspendisse diam imperdiet, porttitor lacinia blandit habitant dolor.
  3. Sagittis habitasse platea elit, imperdiet cum nonummy curabitur.
  4. Porta varius consectetuer pellentesque, quam etiam dui praesent.

Tempus cras praesent blandit urna, accumsan nisl parturient varius dictum, amet mauris non lorem dignissim. Urna molestie ut aenean ipsum, sodales egestas porttitor urna posuere, ut et mauris praesent adipiscing, a consequat neque imperdiet augue. Quis proin sed magna dolor, lorem nulla proin platea, tempor et turpis duis vitae. Ac quisque convallis amet sit, pede risus vulputate nam lectus.

Section header

Malesuada dictum nostra ligula, lacus eget imperdiet pede, eu eleifend tempus amet, nonummy tincidunt lacinia vitae. Harum vel vitae egestas, dictum faucibus convallis lorem, mauris at felis eget, morbi maecenas in interdum accumsan. At nec pede id, aliquet ipsum velit repudiandae.

Diam blandit dui ultrices maecenas, vel nullam amet lorem, vulputate sem sodales egestas condimentum. Etiam ipsum vel nisl, sit interdum ullamcorper nulla, praesent condimentum enim vestibulum, ligula tempus luctus mollis. Nec ut id pellentesque, netus felis diam dui lectus. Lorem leo sed suspendisse dui, sapien perspiciatis aliquam arcu at, adipiscing pede porta quam pede. Molestie imperdiet sed ut, mauris vulputate molestie mauris arcu. Turpis tincidunt purus sit, ipsum auctor orci mi mauris:

  • Lorem ipsum dolor sit amet, sagittis arcu odio dolorem, praesent magna voluptate hendrerit, ullamcorper mollis non dolor.
  • Curabitur in egestas ullamcorper dignissim, lectus arcu suspendisse diam imperdiet, porttitor lacinia blandit habitant dolor.
  • Sagittis habitasse platea elit, imperdiet cum nonummy curabitur.
  • Porta varius consectetuer pellentesque, quam etiam dui praesent.

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