Team:HokkaidoU Japan/Notebook

From 2012.igem.org

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=July=
=July=
==4th==
==4th==
-
Transformation
+
;Transformation
-
*Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
+
#Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
-
**Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43)  
+
#Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated
-
***T7:21hrs Others:20hrs  
+
==5th==
==5th==
-
Transformation
+
;Transformation
-
*Transformation of K346007(Ag43)in DH5α.
+
#Transformation of K346007(Ag43)in DH5α.
-
**Cultivated on LBC in 21hrs.
+
#Cultivated on LBC in 21hrs.
-
Single colony isolation
+
;Single colony isolation
-
*Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
+
#Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
-
**Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins  
+
#Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins  
-
***BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
+
'''BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.'''
==6th==
==6th==
-
Liquid culture
+
;Liquid culture
-
*Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
+
#Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
-
**LB : 1ml is for glycerol stocks and 2ml is for mini-prep.
+
#LB 1ml is for glycerol stocks and 2ml is for mini-prep.
 +
#16hrs Cultivation
-
Single colony isolation
+
;Single colony isolation
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*Single colony isolation of K346007(Ag43).
+
#Single colony isolation of K346007(Ag43).
 +
 
 +
==7th==
 +
;mini-prep
 +
#mini-prep for dT,RBS,pT7 and pLacI-RBS-Ag43.

Revision as of 02:14, 7 July 2012

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Contents

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<) 
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

March

Spring Boot Camp

date
March 5 (Mon) ~ March 9 (Fri)

Monday, March 5

Session #1
Short lecture about moleculer biology (Mr.Yamazaki, our adviser)
Session #2
Tutrial: How to use 'Unipro UGENE' (iTakeshi)
Session #3
Guidance: Wiki Reading (Laury)
Example: 2010 MIT

Tuesday, March 6

Session #4~6
Reading Wikis in turn and discussions
  • 2010 NYU
  • 2009 Cambridge
  • 2009 Growningen

Wednesday, March 7

Session #7~11
Reading Wikis (2)
  • 2010 Washington
  • 2009 Valencia
  • 2011 Barklay
  • 2010 Paris
  • 2010 Bristol

Thursday, March 8

Session #12
2012 Project Brainstorming
The details is secret! :)
Session #13
Guidance: How to read papers (Laury)

Friday, March 9

Session #14
2012 Project Brainstorming (2)
Session #15
Guidance: How to look up papers you want (Laury)
Session #16
Tutorial: Modeling the behavior of cells (iTakeshi)
Session #17
Final Session: Reviewing this camp
Party!!

July

4th

Transformation
  1. Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
  2. Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated

5th

Transformation
  1. Transformation of K346007(Ag43)in DH5α.
  2. Cultivated on LBC in 21hrs.
Single colony isolation
  1. Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
  2. Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins

BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

6th

Liquid culture
  1. Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
  2. LB 1ml is for glycerol stocks and 2ml is for mini-prep.
  3. 16hrs Cultivation
Single colony isolation
  1. Single colony isolation of K346007(Ag43).

7th

mini-prep
  1. mini-prep for dT,RBS,pT7 and pLacI-RBS-Ag43.