Team:HokkaidoU Japan/Notebook

From 2012.igem.org

(Difference between revisions)
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==5th==
==5th==
Transformation
Transformation
-
*Transformation of K346007(Ag43)in DH5α
+
*Transformation of K346007(Ag43)in DH5α.
**Cultivation on LBC.
**Cultivation on LBC.

Revision as of 08:15, 6 July 2012

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Contents

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<) 
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

July

4th

Transformation

  • Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
    • Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43)

5th

Transformation

  • Transformation of K346007(Ag43)in DH5α.
    • Cultivation on LBC.

Single colony isolation

  • Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
    • BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

6th

Liquid culture

  • Liquid culture in LBA(B0015,B0034) and LBC(K542009)
    • LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep