Team:Carnegie Mellon/Notebook/4 June 2012
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- Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. | - Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. |
Revision as of 20:45, 26 June 2012
6/04
- Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
- Harvested DH5-alpha cells from stock culture and made cells competent.
- Followed transformation protocol from Dr. Doonan’s lab (see Protocols)