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Team:Trieste/notebook5
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | We finally obtained positive colonies, checked by PCR, and we cloned it inside the RBS (BBa_B0031). | + | We finally obtained positive colonies of the construct Tse2 toxin-TT_B0015, checked by PCR, and we cloned it inside the RBS (BBa_B0031). As already known in the registry and due to the strong expression of the toxin, we decided to use the weak RBS, because we wanted to avoid that the promoter leakage leads to the destruction of the cell.</br> |
</br> | </br> | ||
| - | We sent the construct to sequence and we waited for the sequencing.</br> | + | We sent the construct to sequence and we waited for the sequencing results.</br> |
</br> | </br> | ||
| - | We also explored another suicide strategy, | + | We also explored another suicide strategy, combining the holin (BBa_K112000)with the LL 37 (BBa_K875009). |
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 17:04, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 5
More
Suicide System
We finally obtained positive colonies of the construct Tse2 toxin-TT_B0015, checked by PCR, and we cloned it inside the RBS (BBa_B0031). As already known in the registry and due to the strong expression of the toxin, we decided to use the weak RBS, because we wanted to avoid that the promoter leakage leads to the destruction of the cell. We sent the construct to sequence and we waited for the sequencing results. We also explored another suicide strategy, combining the holin (BBa_K112000)with the LL 37 (BBa_K875009).Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
