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Team:Trieste/notebook3
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | We inoculated the positive colonies and also checked by digestion | + | We inoculated the positive colonies (RBS_B0034-LL37-TT_B0015) and also checked by digestion. The construct was successfully cloned downstream the inducible promoter T5LacOperator (BBa_K875002). The M15 cells (containing the pREP4 plasmid coding for the Lac repressor) were transformed with the construct described above and seeded in Kan (M15 resistance), Cm (pSB1C3 resistance) in two conditions: with and without IPTG ( the inhibitors of the repressor Lac).</br> |
</br> | </br> | ||
| - | Unfortunately the LL-37 cathelicidin was probably unable to disrupt the cell membrane, as we observed a growth both in induced and not induced.</br> | + | Unfortunately the LL-37 cathelicidin was probably unable to disrupt the cell membrane, as we observed a growth both in induced and not induced culture.</br> |
</br> | </br> | ||
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</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 16:54, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 3
More
Suicide System
We inoculated the positive colonies (RBS_B0034-LL37-TT_B0015) and also checked by digestion. The construct was successfully cloned downstream the inducible promoter T5LacOperator (BBa_K875002). The M15 cells (containing the pREP4 plasmid coding for the Lac repressor) were transformed with the construct described above and seeded in Kan (M15 resistance), Cm (pSB1C3 resistance) in two conditions: with and without IPTG ( the inhibitors of the repressor Lac). Unfortunately the LL-37 cathelicidin was probably unable to disrupt the cell membrane, as we observed a growth both in induced and not induced culture.Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
