Team:Trieste/project/applications

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    <br/>The anti-NoV antibody was also expressed as a SIP
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    <br/><strong>The anti-NoV antibody was also expressed as a SIP
    (Small Immuno Protein) format, which contains the scFv fused to the CH3
    (Small Immuno Protein) format, which contains the scFv fused to the CH3
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    domain of the heavy chain of human immunoglobulin A (IgA). Since the
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    domain of the heavy chain of human immunoglobulin A (IgA)</strong>. Since the
    CH3 region is able to homodimerize, it should confer bivalent binding
    CH3 region is able to homodimerize, it should confer bivalent binding
    properties to the anti-NoV scFv54.6. Secreted SIPs have the dual advantage
    properties to the anti-NoV scFv54.6. Secreted SIPs have the dual advantage
    of being bivalent as full-length antibodies and being small as scFvs. They
    of being bivalent as full-length antibodies and being small as scFvs. They
    have also been shown to have the potential to protect against enteric
    have also been shown to have the potential to protect against enteric
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    infections when administered orally (Bestagno et al 2006). Eventually,
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    infections when administered orally (Bestagno et al 2006). Eventually,
    we planned to produce also the secreted versions of the anti-NoV SIP
    we planned to produce also the secreted versions of the anti-NoV SIP
    54.6 and of the scFv 54.6. Both the scFv and the SIP sequences were
    54.6 and of the scFv 54.6. Both the scFv and the SIP sequences were
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    be released into the extracellular space by passing through porins. This
    be released into the extracellular space by passing through porins. This
    system can also be used for production of small, soluble molecules.
    system can also be used for production of small, soluble molecules.
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Revision as of 23:03, 22 September 2012

Application

More

The safe probiotic constructed here can be used to produce nutritious, preventive or therapeutic molecules. For example, this E.coli can express antitumorals, antibodies against different intestinal pathogens, imunomodulators, antigens (recombinant proteins which act as mucosal vaccine) which are important for the correct development of the immune system etc. It can express also different enzymes as lactase or enzymes needed to recreate the metabolic pathways to produce nutrients such as vitamins.


To give a proof-of-concept, we have used our safe probiotic to deliver to gut mucosa a neutralizing antibody (Ab 54,6) against an emerging Norovirus (NoV), one of the most common causes of gastroenteritis in the world. To this end, we used a LPP-OmpA based cell display systemto express the scFv (single chain fragment variable) format of the antibody attached to the bacterial surface. According to the literature, scFvs anchored to the bacterial surface can bind multiple viral particles and protect efficiently against infection. The chosen scFv sequence, which was already known to inhibit Norovirus (NoV) interaction with cells, was inserted in frame downstream the LPP-OmpA sequence and this construct was cloned under a constitutive promoter. LPP-OmpA is a chimeric sequence having part of the major outer membrane lipoprotein and an outer membrane porin OmpA fragment. This chimeric sequence acts as a leader sequence and an anchor, it transports the scFv fused at its C-terminus through the cytoplasm membrane into the periplasm, where the scFv assumes the right conformation. OmpA portion then introduces itself into the outer membrane displaying extracellularly its C terminus with the Ab attached on.



The anti-NoV antibody was also expressed as a SIP (Small Immuno Protein) format, which contains the scFv fused to the CH3 domain of the heavy chain of human immunoglobulin A (IgA). Since the CH3 region is able to homodimerize, it should confer bivalent binding properties to the anti-NoV scFv54.6. Secreted SIPs have the dual advantage of being bivalent as full-length antibodies and being small as scFvs. They have also been shown to have the potential to protect against enteric infections when administered orally (Bestagno et al 2006). Eventually, we planned to produce also the secreted versions of the anti-NoV SIP 54.6 and of the scFv 54.6. Both the scFv and the SIP sequences were cloned downstream the pelB leader sequence. PelB drives proteins into the periplasmic space, where it is cleaved off. The resulting proteins can be released into the extracellular space by passing through porins. This system can also be used for production of small, soluble molecules.


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