Team:Technion/12 September 2012

From 2012.igem.org

(Difference between revisions)
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==Inbal==
==Inbal==
-
 
+
-Colony PCR for the whole part- pTetO+m-cherry+eT7+terminator within pSB1AK3 (let's call it In1, for my convenience)- I picked randomly 5 colonies, The PCR didn't yield any results.<br>
 +
-I PCRed again pTetO+m-cherry (for the T3 cloning), one sample did actually was amplified, but the other- with the same conditions and PCR and equipment, wasn't amplified (the wonder of science!).<br>
 +
- I minipreped In1 from yestreday and the concentration is 202.5 ng/ul.<br>
 +
- I redid a colony PCR for In1 (again picked 5 colonies by random) but didn't get any good results (not the expected band -3600bp).<br> 
==Asaf==
==Asaf==
I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.<br> I got good DNA concentrations (75.5-120.5 ng/ul).<br>
I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.<br> I got good DNA concentrations (75.5-120.5 ng/ul).<br>

Revision as of 04:01, 20 September 2012



Team:Technion/12_September_2012
March
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      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_March_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_March_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_March_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_March_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_March_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_March_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_March_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_March_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_March_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_March_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_March_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_March_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_March_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_March_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_March_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_March_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_March_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_March_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_March_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_March_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/Team:Technion/21_March_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_March_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_March_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_March_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_March_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_March_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_March_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_March_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_March_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_March_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_March_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
April
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_April_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/2_April_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_April_2012 3] [http://2012.igem.org/Team:Technion/4_April_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_April_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_April_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_April_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_April_2012&preload=Team:Technion/Template:NewDate&action=edit 8]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/9_April_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_April_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_April_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_April_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_April_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/Team:Technion/14_April_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_April_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_April_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_April_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_April_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_April_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_April_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_April_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_April_2012&preload=Team:Technion/Template:NewDate&action=edit 22]
[http://2012.igem.org/Team:Technion/23_April_2012 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_April_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/Team:Technion/25_April_2012 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_April_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_April_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/Team:Technion/28_April_2012 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_April_2012&preload=Team:Technion/Template:NewDate&action=edit 29]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/30_April_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
May
MTWTFSS
  [http://2012.igem.org/Team:Technion/1_May_2012 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_May_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_May_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_May_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_May_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_May_2012&preload=Team:Technion/Template:NewDate&action=edit 6]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/7_May_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_May_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_May_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_May_2012 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_May_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_May_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_May_2012&preload=Team:Technion/Template:NewDate&action=edit 13]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/14_May_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_May_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_May_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_May_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_May_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_May_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_May_2012&preload=Team:Technion/Template:NewDate&action=edit 20]
[http://2012.igem.org/Team:Technion/21_May_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_May_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_May_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_May_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_May_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_May_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_May_2012&preload=Team:Technion/Template:NewDate&action=edit 27]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/28_May_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_May_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_May_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_May_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
June
MTWTFSS
        [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_June_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_June_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_June_2012 3]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/4_June_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/Team:Technion/5_June_2012 5] [http://2012.igem.org/Team:Technion/6_June_2012 6] [http://2012.igem.org/Team:Technion/7_June_2012 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_June_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_June_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_June_2012 10]
[http://2012.igem.org/Team:Technion/11_June_2012 11] [http://2012.igem.org/Team:Technion/12_June_2012 12] [http://2012.igem.org/Team:Technion/13_June_2012 13] [http://2012.igem.org/Team:Technion/14_June_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_June_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_June_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_June_2012 17]
[http://2012.igem.org/Team:Technion/18_June_2012 18] [http://2012.igem.org/Team:Technion/19_June_2012 19] [http://2012.igem.org/Team:Technion/20_June_2012 20] [http://2012.igem.org/Team:Technion/21_June_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_June_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_June_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/Team:Technion/24_June_2012 24]
[http://2012.igem.org/Team:Technion/25_June_2012 25] [http://2012.igem.org/Team:Technion/26_June_2012 26] [http://2012.igem.org/Team:Technion/27_June_2012 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_June_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_June_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_June_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
July
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_July_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/Team:Technion/2_July_2012 2] [http://2012.igem.org/Team:Technion/3_July_2012 3] [http://2012.igem.org/Team:Technion/4_July_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_July_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_July_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_July_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/Team:Technion/8_July_2012 8]
[http://2012.igem.org/Team:Technion/9_July_2012 9] [http://2012.igem.org/Team:Technion/10_July_2012 10] [http://2012.igem.org/Team:Technion/11_July_2012 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_July_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_July_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_July_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_July_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_July_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_July_2012 17] [http://2012.igem.org/Team:Technion/18_July_2012 18] [http://2012.igem.org/Team:Technion/19_July_2012 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_July_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_July_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/Team:Technion/22_July_2012 22]
[http://2012.igem.org/Team:Technion/23_July_2012 23] [http://2012.igem.org/Team:Technion/24_July_2012 24] [http://2012.igem.org/Team:Technion/25_July_2012 25] [http://2012.igem.org/Team:Technion/26_July_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_July_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_July_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/Team:Technion/29_July_2012 29]
[http://2012.igem.org/Team:Technion/30_July_2012 30] [http://2012.igem.org/Team:Technion/31_July_2012 31]
August
MTWTFSS
    [http://2012.igem.org/Team:Technion/1_August_2012 1] [http://2012.igem.org/Team:Technion/2_August_2012 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_August_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/Team:Technion/4_August_2012 4] [http://2012.igem.org/Team:Technion/5_August_2012 5]
[http://2012.igem.org/Team:Technion/6_August_2012 6] [http://2012.igem.org/Team:Technion/7_August_2012 7] [http://2012.igem.org/Team:Technion/8_August_2012 8] [http://2012.igem.org/Team:Technion/9_August_2012 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_August_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_August_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/Team:Technion/12_August_2012 12]
[http://2012.igem.org/Team:Technion/13_August_2012 13] [http://2012.igem.org/Team:Technion/14_August_2012 14] [http://2012.igem.org/Team:Technion/15_August_2012 15] [http://2012.igem.org/Team:Technion/16_August_2012 16] [http://2012.igem.org/Team:Technion/17_August_2012 17] [http://2012.igem.org/Team:Technion/18_August_2012 18] [http://2012.igem.org/Team:Technion/19_August_2012 19]
[http://2012.igem.org/Team:Technion/20_August_2012 20] [http://2012.igem.org/Team:Technion/21_August_2012 21] [http://2012.igem.org/Team:Technion/22_August_2012 22] [http://2012.igem.org/Team:Technion/23_August_2012 23] [http://2012.igem.org/Team:Technion/24_August_2012 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_August_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/Team:Technion/26_August_2012 26]
[http://2012.igem.org/Team:Technion/27_August_2012 27] [http://2012.igem.org/Team:Technion/28_August_2012 28] [http://2012.igem.org/Team:Technion/29_August_2012 29] [http://2012.igem.org/Team:Technion/30_August_2012 30] [http://2012.igem.org/Team:Technion/31_August_2012 31]
September
MTWTFSS
          [http://2012.igem.org/Team:Technion/1_September_2012 1] [http://2012.igem.org/Team:Technion/2_September_2012 2]
[http://2012.igem.org/Team:Technion/3_September_2012 3] [http://2012.igem.org/Team:Technion/4_September_2012 4] [http://2012.igem.org/Team:Technion/5_September_2012 5] [http://2012.igem.org/Team:Technion/6_September_2012 6] [http://2012.igem.org/Team:Technion/7_September_2012 7] [http://2012.igem.org/Team:Technion/8_September_2012 8] [http://2012.igem.org/Team:Technion/9_September_2012 9]
[http://2012.igem.org/Team:Technion/10_September_2012 10] [http://2012.igem.org/Team:Technion/11_September_2012 11] [http://2012.igem.org/Team:Technion/12_September_2012 12] [http://2012.igem.org/Team:Technion/13_September_2012 13] [http://2012.igem.org/Team:Technion/14_September_2012 14] [http://2012.igem.org/Team:Technion/15_September_2012 15] [http://2012.igem.org/Team:Technion/16_September_2012 16]
[http://2012.igem.org/Team:Technion/17_September_2012 17] [http://2012.igem.org/Team:Technion/18_September_2012 18] [http://2012.igem.org/Team:Technion/19_September_2012 19] [http://2012.igem.org/Team:Technion/20_September_2012 20] [http://2012.igem.org/Team:Technion/21_September_2012 21] [http://2012.igem.org/Team:Technion/22_September_2012 22] [http://2012.igem.org/Team:Technion/23_September_2012 23]
[http://2012.igem.org/Team:Technion/24_September_2012 24] [http://2012.igem.org/Team:Technion/25_September_2012 25] [http://2012.igem.org/Team:Technion/26_September_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_September_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_September_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_September_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_September_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
October
MTWTFSS
[http://2012.igem.org/wiki/index.php?title=Team:Technion/1_October_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_October_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_October_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_October_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_October_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_October_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_October_2012&preload=Team:Technion/Template:NewDate&action=edit 7]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/8_October_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_October_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_October_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_October_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_October_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_October_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_October_2012&preload=Team:Technion/Template:NewDate&action=edit 14]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/15_October_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_October_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_October_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_October_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_October_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_October_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_October_2012&preload=Team:Technion/Template:NewDate&action=edit 21]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/22_October_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_October_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_October_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_October_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_October_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_October_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_October_2012&preload=Team:Technion/Template:NewDate&action=edit 28]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/29_October_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_October_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_October_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
November
MTWTFSS
      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_November_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_November_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_November_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_November_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_November_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_November_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_November_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_November_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_November_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_November_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_November_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_November_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_November_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_November_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_November_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_November_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_November_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_November_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_November_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_November_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_November_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_November_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_November_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_November_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_November_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_November_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_November_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_November_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_November_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_November_2012&preload=Team:Technion/Template:NewDate&action=edit 30]


Ilya

- Repeated plate reader for tac+RS+mCherry and tac+RBS+mCherry in pCP. Got similar results to previous experiments. Constitutive expression of mCherry, without any effect of theophylline concentrations. Moreover, the tac+RBS+mCherry gave lower readings than tac+RS+mCherry, in contrast to expectations and one previous experiment. After talking to Roee, we decided to summarize the results along with some calculations and assumptions for the failure of the riboswitch. We will still submit this part to the registry, along with all of our results.
- Prepared a 0.4% gel for the gibson attempts on the phage. Unfortunately, we didn't have time to do the experiments today.
- Began setting up the restrictions for characterizing the MCS plasmid. Shahar and Hila will finish the digestions and run the results on a gel.

Inbal

-Colony PCR for the whole part- pTetO+m-cherry+eT7+terminator within pSB1AK3 (let's call it In1, for my convenience)- I picked randomly 5 colonies, The PCR didn't yield any results.
-I PCRed again pTetO+m-cherry (for the T3 cloning), one sample did actually was amplified, but the other- with the same conditions and PCR and equipment, wasn't amplified (the wonder of science!).
- I minipreped In1 from yestreday and the concentration is 202.5 ng/ul.
- I redid a colony PCR for In1 (again picked 5 colonies by random) but didn't get any good results (not the expected band -3600bp).

Asaf

I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.
I got good DNA concentrations (75.5-120.5 ng/ul).
I cut the Psb1c3 with the MCS with EcoRI and PstI restriction enzymes.
I ran the cut Psb1c3 on gel for extraction. I got the expected 2kb bands.

Shahar cleaned the DNA from the gel I extracted.

Hila

Lior

Noa

Evgeni

Shahar

Rachel