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Team:Wageningen UR/Journal/week10
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'''TuYV''' | '''TuYV''' | ||
| - | * A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. | + | Monday: |
| + | * A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated. | ||
| + | |||
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* Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts. | * Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts. | ||
Revision as of 09:18, 18 September 2012
week 10: 2 july - 8 july
Office work
Lab work
TuYV
Monday:
- A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated.
- Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.
Written by: Wouter
