Team:Frankfurt/Notebook

From 2012.igem.org

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!* contains no His, Leu, Trp and Uracil <br> ** addition of this components depents on the respective selection medium
!* contains no His, Leu, Trp and Uracil <br> ** addition of this components depents on the respective selection medium
 +
{|class=wikitable float:right
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|-
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!colspan=4|SOC-Medium for Regeneration of transformed ''Escherichia coli`s'' after Electroporation
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|-
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|Trypton||2 % (w/v)
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|-
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|Yeast Extract||0.5 % (w/v)
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|-
 +
|NaCl||10 mM
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|-
 +
|KCl||2,5 mM
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|-
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|MgCl<sub>2</sub>||10 mM
 +
|-
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|MgSO<sub>4</sub>||10 mM
 +
|-
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|Glucose||20 mM
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|}

Revision as of 13:52, 14 September 2012

Frankfurt logo.png
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Methods and Protocols

Culture Medium

Full Medium (YEPD) for Yeast
Yeast Extract1 % (weight/volume)
Pepton2 % (w/v)
Glucose2 % (w/v)
Synthetic Complete Medium (SC) for Yeast
Yeast Nitrogen Base0.17 % (w/v)
Ammoniumsulfate0.5 % (w/v)
Amino Acid Mix*50 ml/l
Histidin**0.25 mM
Tryptophan**0.19 mM
Leucin**0.35 mM
Uracil**0.44 mM

!* contains no His, Leu, Trp and Uracil
** addition of this components depents on the respective selection medium

SOC-Medium for Regeneration of transformed Escherichia coli`s after Electroporation
Trypton2 % (w/v)
Yeast Extract0.5 % (w/v)
NaCl10 mM
KCl2,5 mM
MgCl210 mM
MgSO410 mM
Glucose20 mM
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