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Team:Frankfurt/Notebook
From 2012.igem.org
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. | You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. | ||
| + | |||
| + | ==Methods and Protocols== | ||
| + | =Culture Medium= | ||
| + | {|class=wikitable float:right | ||
| + | |- | ||
| + | !colspan=4|Full Medium (YEPD) for Yeast | ||
| + | |- | ||
| + | |Yeast Extract||1 % (weight/volume) | ||
| + | |- | ||
| + | |Pepton||2 % (w/v) | ||
| + | |- | ||
| + | |Glucose||2 % (w/v) | ||
| + | |} | ||
| + | |||
| + | {|class=wikitable float:right | ||
| + | |- | ||
| + | !colspan=4|Synthetic Complete Medium (SC) for Yeast | ||
| + | |- | ||
| + | |Yeast Nitrogen Base||0.17 % (w/v) | ||
| + | |- | ||
| + | |Ammoniumsulfate||0.5 % (w/v) | ||
| + | |- | ||
| + | |Amino Acid Mix*||50 ml/l | ||
| + | |- | ||
| + | |Histidin**||0.25 mM | ||
| + | |- | ||
| + | |Tryptophan**||0.19 mM | ||
| + | |- | ||
| + | |Leucin**||0.35 mM | ||
| + | |- | ||
| + | |Uracil**||0.44 mM | ||
| + | |} | ||
| + | |||
| + | !* contains no His, Leu, Trp and Uracil <br> ** addition of this components depents on the respective selection medium | ||
Revision as of 13:33, 14 September 2012
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|---|
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Methods and Protocols
Culture Medium
| Full Medium (YEPD) for Yeast | |||
|---|---|---|---|
| Yeast Extract | 1 % (weight/volume) | ||
| Pepton | 2 % (w/v) | ||
| Glucose | 2 % (w/v) | ||
| Synthetic Complete Medium (SC) for Yeast | |||
|---|---|---|---|
| Yeast Nitrogen Base | 0.17 % (w/v) | ||
| Ammoniumsulfate | 0.5 % (w/v) | ||
| Amino Acid Mix* | 50 ml/l | ||
| Histidin** | 0.25 mM | ||
| Tryptophan** | 0.19 mM | ||
| Leucin** | 0.35 mM | ||
| Uracil** | 0.44 mM | ||
!* contains no His, Leu, Trp and Uracil
** addition of this components depents on the respective selection medium
