Team:LMU-Munich/Spore Coat Proteins/mainresult

From 2012.igem.org

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===GFP as a Proof of Principle===
===GFP as a Proof of Principle===
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<p align="justify">We designed the first '''Sporo'''bead to express GFP to evaluate our system and compare different [[Team:LMU-Munich/Spore_Coat_Proteins/cloning|constructs]]. We had to ensure that production is both high and takes place on the edge of spores. The following graph (Fig. 6) shows the microscopy pictures and analysis of the strongest of the five constructs integrated into wildtype W168 (B53) and the deletion strain B 49 (B70).</p>   
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<p align="justify">We designed the first '''Sporo'''bead to express GFP to evaluate our system and compare different [[Team:LMU-Munich/Spore_Coat_Proteins/cloning|constructs]].
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This two short time lapse videos shows the formation of a spore and lollowing lysis of the mother cell of one of our constructs.
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<iframe width="315" height="315" src="http://www.youtube.com/embed/fXhUsB4dTIs" frameborder="0" allowfullscreen></iframe></div>
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<iframe width="315" height="315" src="http://www.youtube.com/embed/XrfTY2Lsc-Q" frameborder="0" allowfullscreen></iframe></div>
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We had to ensure that production is both high and takes place on the edge of spores. The following graph (Fig. 6) shows the microscopy pictures and analysis of the strongest of the five constructs integrated into wildtype W168 (B53) and the deletion strain B 49 (B70).</p>   
{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
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Revision as of 15:20, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU eppis.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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