Team:Exeter/lab book/3gip/wk6

From 2012.igem.org

(Difference between revisions)
m
Line 99: Line 99:
   <td width="850" height="250">
   <td width="850" height="250">
   <!------------INSERT WEEKLY IMAGE HERE------------>
   <!------------INSERT WEEKLY IMAGE HERE------------>
-
     <img src="https://static.igem.org/mediawiki/2012/archive/a/ac/20120925181043%21Exe2012Fred_in_lab2.jpg" alt="" title="" width="850" height="250">
+
     <img src="https://static.igem.org/mediawiki/2012/2/27/Exe2012FreddieInTheLab.JPG" alt="" title="" width="850" height="250">
   </td>
   </td>
   </tr>
   </tr>

Revision as of 10:00, 26 September 2012

ExiGEM2012 Lab Book 3GP wk6

The 3-Gene Inducible Plasmid: 13th - 17th August 2012

Monday 13th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Tuesday 14th August

See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005)


Wednesday 15th August

Morning

•Miniprepping

BBa_J13002 + wbnK BBa_B0014 in pSB1T3

wfcA + BBa_B0014 in pSB1T3

wbnJ + BBa_B0014 in pSB1T3

BBa_B0034 wbbC + BBa_B0014 in pSB1T3

Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed.

After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation.

40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased.

All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations.


Wednesday 15th August – Friday 17th August

Preparation for presentation so no lab work.

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/3gip/wk6"