Team:LMU-Munich/Data

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[[File:Data banner.resized WORDS.JPG|620px|link=]]
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==Data==
 
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On this page, we will upload data to explain our project progress. More to come!!!
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[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
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<br>
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<div class="box" style= "background-color:#e4f1d7">
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Or see the results pages of our projects to follow our progress:
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==='''''Bacillus''B'''io'''B'''rick'''B'''ox===
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{|
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====Evaluation of ''B. subtilis'' vectors====
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.</p>
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|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Vectors]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
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</div>
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
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<div class="box">
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</a>
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====Anderson Promoter Evaluation====
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<font size="1">
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{| "width=100%" style="text-align:center;"|
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</font>
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|<p align="justify">Eleven Anderson promoters were measured in ''B. subtilis'' and showed relatively weak activity.</p>
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<br />
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|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction"><font size="1" face="verdana">Bacillus<BR>Intro</font></a>
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]
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====Constitutive ''Bacillus'' Promoters====
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|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
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|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Constitutive]]
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<div class="box">
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====Inducible ''Bacillus'' Promoters====
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|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
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|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Inducible]]
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</div>
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|<html>
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[[File:SporeCoat.png|90px|right|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
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<br>
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<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=70"/>
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<div class="box" style= "background-color:#e4f1d7">
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</a>
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=='''Sporo'''beads==
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</div>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
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|<html>
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<div class="box">
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
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===Crust Promoter Evaluation===
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<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=70"/>
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
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<font size="1">
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|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
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</font>
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/crustpromoters]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
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</div>
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|<html>
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<div class="box">
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
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===GFP-'''Sporo'''bead Evaluation===
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<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=70"/>
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{| "width=100%" style="text-align:center;"|
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</a>
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|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.</p>
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<font size="1">
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|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/gfp_spore]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
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<div class="box">
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==='''Sporo'''bead Purification===
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|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysozyme is the best!</p>
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|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Sporepurification]]
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|}
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</div>
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[[File:Auswertung Anderson promoters.png|thumb|right|Figure 1: luminescence measurement of Anderson promoters in the reporter vector pSB<sub>''Bs''</sub>3C-''luxABCDE''. OD''600'' (right), LUMI (center) and OD''600'' per LUMI (left)depending on the time (h). Data come from three independant experiments. Curves were fitted over each other (t=0, OD''600''=0,3) and smothed by taking average of three neighbouring points.]]
 
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Abb. 5: Lumineszenzmessung der Anderson-Promotoren. OD600 (links), Lumineszenz (mitte), Lumineszenz pro OD600 (rechts) der Anderson-Promotoren J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 (von oben nach unten) in Abhängigkeit der Zeit (h). Dabei wurden drei unabhängige Experimente einbezogen. Zur verbesserten Darstellung wurde der Mittelwert dreier nebeneinanderliegender Zeitpunkte gebildet und die Kurven in x-Richtung verschoben, sodass für alle Promotoren zum Zeitpunkt t=0 die OD600 0,1 war.
 
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[[File:GerminationSTOP.png|90px|right|link=Team:LMU-Munich/Germination_Stop]]
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<div class="box" style= "background-color:#e4f1d7">
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==='''Germination'''STOP===
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</div>
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<div class="box">
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===Knockouts of germination genes ===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">We successfully prevented spore germination by multiple gene knockouts.</p>
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|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Knockout]]
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<div class="box">
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==='''Suicide'''Switch ===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.</p>
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|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Suicideswitch]]
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|}
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</div>
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[[File:LMU-Munich-Invertersign.png|90px|right|link=Team:LMU-Munich/Inverter]]
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<br>
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<div class="box" style= "background-color:#e4f1d7">
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===Inverter===
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</div>
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===β-galactosidase assay of Inverter with ''lacZα'' ===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">It works! Invert the output of your promoter of choice.</p>
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|[[File:LMU Inverter graph.png|200px|link=Team:LMU-Munich/Data/Inverter]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Inverter]]
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|}
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</div>
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<div class="box" style= "background-color:#e4f1d7">
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==='''Trash'''can===
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Things that were a pain in the ''neck''!
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</div>
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<div class="box">
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===''cge''A and P<sub>''cge''A</sub>===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">
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Unexpected restriction sites combined with no activity at all - what a bummer!</p>
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|[[File:LMU cgea preview.jpg|200px|link=Team:LMU-Munich/Data/cgeA]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/cgeA]]
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|}
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</div>
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<div class="box">
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===MazF===
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{| "width=100%" style="text-align:center;"|
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|<p align="justify">
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The story of trying to clone a toxin from ''E. coli'' in ''E. coli'': They die, dammit! (...not a surprise, really...)
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</p>
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|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/MazF]]
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|}
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</div>
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<div class="box">
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===Xylose promoters===
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|<p align="justify">
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All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).
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</p>
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|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Pxyl]]
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|}
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</div>
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<p></p>
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For our big breakthroughs, or to follow specific projects, see the individual project pages:
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<div class="box">
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====Project Navigation====
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|-
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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|}
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</div>
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{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 14:31, 22 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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