Team:Carnegie Mellon/Notebook/5 June 2012
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- | '''June 05, 2012:''' | + | '''June 05, 2012: 09:30 ''' |
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- | + | == Observations of Plates == | |
* Done after spreading transformed cells on LB+Amp plates overnight | * Done after spreading transformed cells on LB+Amp plates overnight | ||
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- | [[File:Carnegie_05_June_2012_im1.jpg | LB+Amp Control Groups | | + | [[File:Carnegie_05_June_2012_im1.jpg | LB+Amp Control Groups | border | 500px]] |
- | <br/> | + | <br/><br/> |
- | [[File:Carnegie_05_June_2012_im2.jpg | LB+Amp Charlie Group | | + | [[File:Carnegie_05_June_2012_im2.jpg | LB+Amp Charlie Group | border | 500px]] |
* Note: Charlie only had 1 colony, photo was taken after picking up colony | * Note: Charlie only had 1 colony, photo was taken after picking up colony | ||
* Taken using Red Gel Documentation System from Westburg | * Taken using Red Gel Documentation System from Westburg | ||
+ | |||
+ | |||
+ | === Comments: === | ||
+ | Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected. | ||
+ | |||
+ | |||
+ | <font size=5> | ||
+ | '''June 05, 2012: 16:00 ''' | ||
+ | </font> | ||
+ | |||
+ | == Inoculation of cells == | ||
+ | * Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media | ||
+ | * Left in 37 degree incubator for incubation till next morning | ||
+ | |||
+ | === Comments: === | ||
+ | None |
Latest revision as of 23:05, 26 June 2012
June 05, 2012: 09:30
Contents |
Observations of Plates
- Done after spreading transformed cells on LB+Amp plates overnight
Name | #Colonies: Plate 1 | #Colonies: Plate 2 |
---|---|---|
Charlie | 1 | 0 |
dNP138 | 7 | 3 |
- Control | 0 | 0 |
+ Control | TMTC (>200) | TMTC (>200) |
- Note: Charlie only had 1 colony, photo was taken after picking up colony
- Taken using Red Gel Documentation System from Westburg
Comments:
Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.
June 05, 2012: 16:00
Inoculation of cells
- Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
- Left in 37 degree incubator for incubation till next morning
Comments:
None