Team:Carnegie Mellon/Notebook/5 June 2012
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- | '''June 05, 2012:''' | + | <font size=5> |
- | + | '''June 05, 2012: 09:30 ''' | |
- | + | </font> | |
+ | == Observations of Plates == | ||
+ | * Done after spreading transformed cells on LB+Amp plates overnight | ||
- | + | {| class="wikitable" border=1 align=left style="text-align: center;" | |
- | {| class="wikitable" style="text-align: center;" | + | |+ Plate Observations |
|- | |- | ||
! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2 | ! Name !! #Colonies: Plate 1 !! #Colonies: Plate 2 | ||
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|} | |} | ||
+ | <br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
+ | [[File:Carnegie_05_June_2012_im1.jpg | LB+Amp Control Groups | border | 500px]] | ||
+ | <br/><br/> | ||
+ | [[File:Carnegie_05_June_2012_im2.jpg | LB+Amp Charlie Group | border | 500px]] | ||
+ | * Note: Charlie only had 1 colony, photo was taken after picking up colony | ||
+ | * Taken using Red Gel Documentation System from Westburg | ||
+ | === Comments: === | ||
+ | Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected. | ||
+ | <font size=5> | ||
+ | '''June 05, 2012: 16:00 ''' | ||
+ | </font> | ||
- | + | == Inoculation of cells == | |
- | + | * Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media | |
- | + | * Left in 37 degree incubator for incubation till next morning | |
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- | + | === Comments: === | |
- | + | None | |
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Latest revision as of 23:05, 26 June 2012
June 05, 2012: 09:30
Contents |
Observations of Plates
- Done after spreading transformed cells on LB+Amp plates overnight
Name | #Colonies: Plate 1 | #Colonies: Plate 2 |
---|---|---|
Charlie | 1 | 0 |
dNP138 | 7 | 3 |
- Control | 0 | 0 |
+ Control | TMTC (>200) | TMTC (>200) |
- Note: Charlie only had 1 colony, photo was taken after picking up colony
- Taken using Red Gel Documentation System from Westburg
Comments:
Successful transformation, negative control has none, positive control has TMTC. However, plasmid concentrations/purity are significantly lower than expected.
June 05, 2012: 16:00
Inoculation of cells
- Took 1 colony of cells from DNP and Charlie respectively and inoculated in 3 ml of LB+Amp media
- Left in 37 degree incubator for incubation till next morning
Comments:
None