Team:Paris Bettencourt/Suicide

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Overview

Our goal is to engineer a synthetic toxin-antitoxin system from the wild type colicin E2. This synthetic toxin-antitoxin system is species specific, will allow for population level cell death, destruction of the genetic material, and will function on a tunable delay.

Objectives

1. Design an anti-toxin part that confers immunity against the colicin E2 toxin
2. Develop RFP labeled MG1655 Z1 strains immune to colicin E2 toxin
3. Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of WT ColE2 cells
4. Design a colicin E2 toxin part under the control of a constitutive promoter and establish in immune MG1655 Z1 cells to generate synthetic ColE2 cells
5. Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of synthetic ColE2 cells
6. Tune the concentrations of toxin and anti-toxin
7. Combine our system with the Restriction Enzyme Group`s work, measure viability

Design

Present the design of your system, both in a written form, and a schematic one.

Experiments and results

Characterisation of X

Experimental setup

Describe the experiment

Results

Present your results

Testing of the system

Experimental setup

Describe the experiment

Results

Present your results