Team:WHU-China/HumanPractice

From 2012.igem.org

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(15 intermediate revisions not shown)
Line 15: Line 15:
Home : [
Home : [
{
{
-
title : "<strong>what's new</strong>",
+
title : "<strong>What's New</strong>",
picArray : [
picArray : [
-
new picObj("http://imgsrc.baidu.com/forum/pic/item/d25e8bd4b31c87011d9cdb7c277f9e2f0708ff34.jpg", "one piece", "yi zhang haizeiwang de tu", "http://tieba.baidu.com/f?kw=%BA%A3%D4%F4%CD%F5"),
+
new picObj("https://static.igem.org/mediawiki/2012/e/e3/What%27s_new_first.png","Overall Project", "Compared with traditional methods of losing weight, our probiotic-- E.coslim has three advantages: monitoring, high efficiency and long term efficiency.", "https://2012.igem.org/Team:WHU-China/Project?catalog=0"),
-
new picObj("http://imgsrc.baidu.com/forum/pic/item/d25e8bd4b31c87011d9cdb7c277f9e2f0708ff34.jpg", "one piece", "yi zhang haizeiwang de tu", "http://tieba.baidu.com/f?kw=%BA%A3%D4%F4%CD%F5"),
+
new picObj("https://static.igem.org/mediawiki/2012/f/ff/Promoter_design.png", "Monitoring", "With two novel synthetic promoters, Pcar and PfadR, our E.coslim is able to sense and respond to the high calorie molecules.", "https://2012.igem.org/Team:WHU-China/Project?catalog=2#Pcar"),
-
new picObj("http://imgsrc.baidu.com/forum/pic/item/d25e8bd4b31c87011d9cdb7c277f9e2f0708ff34.jpg", "one piece", "yi zhang haizeiwang de tu", "http://tieba.baidu.com/f?kw=%BA%A3%D4%F4%CD%F5")
+
new picObj("http://partsregistry.org/wiki/images/f/ff/%E5%85%A8%E6%99%AF%E5%8A%A0%E6%AD%BB%E4%BA%A11.png", "Becoming highly efficient-- Panorama of the All Devices", "Four novel devices-- Fatty Acid Degradation, Cellulose Synthesis, Colonization and Death were designed to ensure high efficiency respond to high- calorie molecule, making people slim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=3"),
 +
          new picObj("https://static.igem.org/mediawiki/2012/0/09/%E8%83%96%E5%AD%90%E7%98%A6%E5%AD%90.png", "Long term efficiency of the E.coslim", "By mathematical model, we showed that when viewed at a macroscopic level, the E. coslim may achieve long term efficiency by modulating the composition of gut microbiota.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=2")
 +
]
]
},
},
{
{
-
title : "<strong>pathway introduction</strong>",
+
title : "<strong>Pathway Introduction</strong>",
picArray : [
picArray : [
-
new picObj("2.png", "title 2", "pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1pathway introduction 1production 1", "#"),
+
new picObj("https://static.igem.org/mediawiki/igem.org/8/82/Pathway_FattyAcid.png", "Fatty Acid Degradation", "Feel free to eat meat since the fat is all degraded by our E.coslim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=3"),
-
new picObj("2.png", "title 2", "pathway introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2 introduction 2", "#"),
+
new picObj("https://static.igem.org/mediawiki/igem.org/1/18/Pathway_Cellulose.png", "Cellulose Synthesis", "Transforming glucose into cellulose, the high-sugar food you eat will be turned into vegetables!", "https://2012.igem.org/Team:WHU-China/Project?catalog=4"),
-
new picObj("2.png", "title 2", "pathway introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3way introduction 3", "#")
+
new picObj("https://static.igem.org/mediawiki/2012/e/ea/Device_three.png", "Colonization", "The magic molecule c-di-GMP enables E.coslim colonizing in the gut to better fulfill its magic function!", "https://2012.igem.org/Team:WHU-China/Project?catalog=5")
]
]
},
},
 +
{
{
-
title : "<strong>future prospective</strong>",
+
title : "<strong>Future Perspective</strong>",
picArray : [
picArray : [
-
new picObj("3.png", "title 3", "future prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1uture prospective 1utureive 1", "#"),
+
new picObj("https://static.igem.org/mediawiki/2012/a/ab/%E5%8F%8C%E8%83%B6%E5%9B%8A2.jpg", "Two Capsule Design", "Capsule I, the E.coslim, makes you slim, while Capsule II, xylose, terminates the effects of the former.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
-
new picObj("3.png", "title 3", "future prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospective 2e prospecpective 2", "#"),
+
new picObj("https://static.igem.org/mediawiki/2012/d/d2/Fpbody2.jpg", "In vitro experiment in a simulated intestine", "The gut microbiota and E.coslim will be inoculated in a simulated intestine, then the concentration measurement of each component will tell us whether E.coslim works as desired.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
-
new picObj("3.png", "title 3", "future prospective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3pective 3", "#")
+
new picObj("https://static.igem.org/mediawiki/2012/8/85/%E5%B0%8F%E9%BC%A0%E5%AE%9E%E9%AA%8C02.jpg", "In vivo experiment in mice", "E.coslim will also be inoculated into mice and the culture-independent microbiome analysis will tell us the real situation in vivo.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0")
 +
]
 +
},
 +
 
 +
 
 +
 
 +
{
 +
title : "<strong>Achievements</strong>",
 +
picArray : [
 +
new picObj("https://static.igem.org/mediawiki/2012/d/d1/Achievements_device_xia.png", "Devices", "This year, our project has focused on some of the hotspot issues, such as c-di-CMP, gut microbiota. In all we submitted 66 new biobricks and 39 of them have been proved to be effective.", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 
 +
new picObj("https://static.igem.org/mediawiki/2012/c/ca/Achievements_1.png", "Biobicks we have improved", "In our project, we have improved the function of some biobricks,such as BBa_J54222, BBa_K188011 and BBa_R0051. ", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 
 +
new picObj("https://static.igem.org/mediawiki/2012/f/f5/Achievements_2.png", "Novel promoter we designed", "As the existing promoter can not meet our demand, we designed two novel promoters Pcar and Pfad to achieve the goal. In addition, we also have set up a standard for promoter design.", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 
 +
new picObj("https://static.igem.org/mediawiki/2012/2/27/Methods_we_improve.png", "Methods we improved", "In our project,we improved some traditional methods to measure the comsumption of fatty acids and production of cellulose, and also we developed a software named FANCY to detect the fluorescence intensity of single cell. ", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 
 +
new picObj("https://static.igem.org/mediawiki/2012/7/79/Human_practice_xia.png", "Human Practice", "We have helped HUST iGEM team debug their project and mathematical model. We also did a survey on GMOs and publicized iGEM to the public.", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
]
]
},
},
{
{
-
title : "<strong>about us</strong>",
+
title : "<strong>About Us</strong>",
picArray : [
picArray : [
-
new picObj("4.png", "title 4", "about us 1", "#"),
+
new picObj("https://static.igem.org/mediawiki/2012/a/a7/Whu-2012-1.jpg", "WHU-China 2012, I was there", "The Entrance of State Wuhan University", "https://2012.igem.org/Team:WHU-China/Team?catalog=1"),
-
new picObj("4.png", "title 4", "about us 2", "#"),
+
new picObj("https://static.igem.org/mediawiki/2012/2/23/Whu2012heying.jpg", "MIT, we're coming!", "Collge of Life Sciences of WHU", "https://2012.igem.org/Team:WHU-China/Team?catalog=4"),
-
new picObj("4.png", "title 4", "about us 3", "#")
+
new picObj("https://static.igem.org/mediawiki/2012/4/43/Whu2012-2.jpg", "WHU-China, Hooray!", "Yuntai Mountain, Henan Province", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=5")
]
]
}
}
Line 49: Line 70:
Team : [
Team : [
{
{
 +
title : '<strong>Team Introduction</strong>',
 +
dir : []
 +
},
 +
                {
title : '<strong>Members</strong>',
title : '<strong>Members</strong>',
dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'Jiawei Huang',
-
href : '#aaaa'
+
href : '#Jiawei Huang'
 +
},
 +
{
 +
title : 'Kuanwei Sheng',
 +
href : '#Kuanwei Sheng'
 +
},
 +
{
 +
title : 'Xian Xia',
 +
href : '#Xian Xia'
 +
},
 +
{
 +
title : 'Long Mei',
 +
href : '#Long Mei'
 +
},
 +
{
 +
title : 'Fan Cheng',
 +
href : '#Fan Cheng'
 +
},
 +
{
 +
title : 'Wenxiong Zhou',
 +
href : '#Wenxiong Zhou'
 +
},
 +
{
 +
title : 'Boxiang Wang',
 +
href : '#Boxiang Wang'
 +
},
 +
{
 +
title : 'Tong Wang',
 +
href : '#Tong Wang'
 +
},
 +
{
 +
title : 'Jiani Hu',
 +
href : '#Jiani Hu'
 +
},
 +
{
 +
title : 'Shiwei Gao',
 +
href : '#Shiwei Gao'
 +
},
 +
{
 +
title : 'Jing Zhang',
 +
href : '#Jing Zhang'
 +
},
 +
                        {
 +
title : 'Yuan Feng',
 +
href : '#Yuan Feng'
 +
},
 +
 
 +
{
 +
title : 'Pengcheng Zhou',
 +
href : '#PengchengZhou'
 +
},
 +
{
 +
title : 'Chang Liu',
 +
href : '#Chang Liu'
 +
},
 +
{
 +
title : 'Min Ye',
 +
href : '#Min Ye'
 +
},
 +
{
 +
title : 'Xuepeng Huang',
 +
href : '#Xuepeng Huang'
 +
},
 +
{
 +
title : 'Yancheng Liu',
 +
href : '#Yancheng Liu'
 +
},
 +
{
 +
title : 'Cheng Zhao',
 +
href : '#Cheng Zhao'
 +
},
 +
{
 +
title : 'Chao Huang',
 +
href : '#Chao Huang'
 +
},
 +
                        {
 +
title : 'Yutian Zhang',
 +
href : '#Yutian Zhang'
 +
},
 +
 
 +
{
 +
title : 'Wei Hong',
 +
href : '#Wei Hong'
 +
},
 +
{
 +
title : 'Liuyi Hu',
 +
href : '#Liuyi Hu'
 +
                                                },
 +
{
 +
title : 'Jin Liu',
 +
href : '#Jin Liu'
}
}
-
]
+
      ]
},
},
{
{
Line 61: Line 176:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Jianbo Wang',
-
href : '#bbbb'
+
href : '#Jianbo Wang'
-
}
+
},
 +
                                                {
 +
                                                        title : 'Zhixiong Xie',
 +
                                                        href : '#Zhixiong Xie'
 +
                                                },
 +
                                                {
 +
                                                        title : 'Yu Chen',
 +
                                                        href : '#Yu Chen'
 +
                                                }
]
]
},
},
Line 71: Line 194:
},
},
{
{
-
title : '<strong>about WHU</strong>',
+
title : '<strong>About WHU</strong>',
dir : []
dir : []
},
},
{
{
-
title : '<strong>about Our Lab</strong>',
+
title : '<strong>About Our Lab</strong>',
dir : []
dir : []
},
},
{
{
-
title : '<strong>Acknol-</br>edgements</strong>',
+
title : '<strong>Acknow-</br>ledgements & Attributions</strong>',
-
dir : []
+
dir : [
-
},
+
    {
 +
title : 'Acknowledgements',
 +
href : '#Acknowledgements'
 +
},
 +
                        {
 +
                            title : 'Attributions',
 +
                            href : '#Attributions'
 +
                        },
 +
]
 +
}
],
],
-
Project : [
+
Project: [{
-
{
+
title: '<strong>Description</strong>',
-
title : '<strong>Outline</strong>',
+
dir: []
-
dir : [
+
-
{
+
-
title : 'aaaa',
+
-
href : '#aaaa'
+
-
}
+
-
]
+
},
},
{
{
-
title : '<strong>Background</strong>',
+
title: '<strong>Background</strong>',
-
dir : [
+
dir: [
-
{
+
 
-
title : 'bbbb',
+
-
href : '#bbbb'
+
-
}
+
]
]
},
},
{
{
-
title : '<strong>Promoter Design</strong>',
+
title: '<strong>Promoter Design</strong>',
-
dir : []
+
dir: [{
 +
title: 'Introduction',
 +
href: '#Introduction'
 +
},{
 +
title: 'Indirect Pathway Design',
 +
href: '#Indirect Pathway Design'
 +
},
 +
{
 +
title: 'Direct Regulatory Promoter Design',
 +
href: '#Direct Regulatory Promoter Design'
 +
},
 +
{
 +
title: '<i>Pcar</i>',
 +
href: '#Pcar'
 +
},
 +
{
 +
title: '<i>PfadR</i>',
 +
href: '#PfadR'
 +
},
 +
{
 +
title: 'Standard',
 +
href: '#Standard'
 +
}]
},
},
{
{
-
title : '<strong>Device I: Fatty Acid Degradation</strong>',
+
title: '<strong>Device I: Fatty Acid Degradation</strong>',
-
dir : []
+
dir: [
 +
{
 +
    title: 'Purpose',
 +
href: '#Purpose'
 +
},
 +
{
 +
    title: 'Outline',
 +
    href: '#Outline'
 +
},
 +
{
 +
    title: 'Progress',
 +
    href: '#Progress'
 +
},
 +
{
 +
    title: 'Experimental Procedure',
 +
    href: '#Experimental Procedure'
 +
},
 +
{
 +
    title: 'Results',
 +
    href: '#Results'
 +
}
 +
]
},
},
{
{
-
title : '<strong>Device II: Cellulose Synthesis</strong>',
+
title: '<strong>Device II: Cellulose Synthesis</strong>',
-
dir : []
+
dir: [{
 +
    title: 'Outline',
 +
href: '#Outline1'
 +
},
 +
{
 +
    title: 'Design',
 +
href: '#Design1'
 +
},
 +
{
 +
    title: 'Progress',
 +
href: '#Progress1'
 +
},
 +
{
 +
    title: 'Results',
 +
href: '#Results1'
 +
},{
 +
    title: 'Discussion',
 +
href: '#Discussion1'
 +
},]
},
},
{
{
-
title : '<strong>Device III: Colonization</strong>',
+
title: '<strong>Device III: Colonization</strong>',
-
dir : []
+
dir: [
 +
{
 +
    title: 'Purpose',
 +
href: '#Purpose2'
 +
},
 +
{
 +
    title: 'Outline',
 +
    href: '#Outline2'
 +
},
 +
{
 +
    title: 'Experimental Procedure',
 +
    href: '#Experimental Procedure2'
 +
},
 +
{
 +
    title: 'Results',
 +
    href: '#Results2'
 +
}
 +
]
},
},
-
],
+
{
-
Standard : [
+
title: '<strong>Device IV: Death</strong>',
 +
dir: [{
 +
    title: 'Outline',
 +
    href: '#Outline4'
 +
},
 +
{
 +
    title: 'Description',
 +
    href: '#Description'
 +
},
 +
 +
{
 +
    title: 'Prevention of HGT',
 +
    href: '#Prevention of HGT'
 +
}]
 +
},
 +
 
 +
],
 +
Modeling : [
{
{
-
title : '<strong>Future Perspectives</strong>',
+
title : '<strong>Future Perspective</strong>',
dir : []
dir : []
},
},
Line 130: Line 348:
dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'The Ordinary Differential Equations of the Model',
-
href : '#aaaa'
+
href : '#The Ordinary Differential Equations of the Model'
 +
},
 +
{
 +
title : 'Analysis on the Steady State of the ODE',
 +
href : '#Analysis on the Steady State of the ODE'
 +
},
 +
{
 +
title : 'Parameter Screening',
 +
href : '#Parameter Screening'
 +
},
 +
{
 +
title : 'Conclusion',
 +
href : '#Conclusion'
}
}
]
]
Line 139: Line 369:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Gut Microbiota Regulation',
-
href : '#bbbb'
+
href : '#Gut Microbiota Regulation'
 +
},
 +
{
 +
title : 'Situation Before the Establishment of <i>E. coslim</i> Flora',
 +
href : '#Situation Before the Establishment of <i>E. coslim</i> Flora'
 +
},
 +
{
 +
title : 'Situation After the Establishment of <i>E. coslim</i> Flora',
 +
href : '#Situation After the Establishment of <i>E. coslim</i> Flora'
 +
},
 +
{
 +
    title : 'Conclusion',
 +
href : '#Conclusion1'
}
}
]
]
 +
},
},
{
{
title : '<strong>Software Tool: FANCY</strong>',
title : '<strong>Software Tool: FANCY</strong>',
-
dir : []
+
dir : [
 +
{
 +
title : 'Abstract',
 +
href : '#Abstract'
 +
},
 +
{
 +
title : 'Protocol',
 +
href : '#Protocol'
 +
},
 +
{
 +
title : 'Performance Evaluation',
 +
href : '#Performance Evaluation'
 +
},
 +
{
 +
title : 'Source Code',
 +
href : '#Source Code'
 +
}
 +
                                              ]
}
}
],
],
-
Notes : [
+
Notebooks : [
 +
                {
 +
title : '<strong>Achievements</strong>',
 +
dir : []
 +
},
 +
{
 +
title : '<strong>Parts</strong>',
 +
dir : []
 +
},
{
{
title : '<strong>Safety</strong>',
title : '<strong>Safety</strong>',
dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'Brief Answers to Safety Questions',
-
href : '#aaaa'
+
href : '#Brief Answers to Safety Questions'
 +
},
 +
{
 +
    title : 'General Safety Issues',
 +
href : '#General Safety Issues'
 +
},
 +
{
 +
    title : 'Safety Considerations of Our Biobrick parts and Our Project',
 +
href : '#Safety Considerations of Our Biobrick parts and Our Project'
 +
},
 +
{
 +
    title : 'Safety Management and Practice',
 +
href : '#Safety Management and Practice'
 +
},
 +
{
 +
    title : 'Biosafety and Publicity',
 +
href : '#Biosafety and Publicity'
}
}
]
]
Line 163: Line 447:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Protocol for Fluorescence Measurement',
-
href : '#bbbb'
+
href : '#Protocol for fluorescence measurement'
 +
},
 +
{
 +
title : 'Protocol of Cupric-Soap Reaction',
 +
href : '#Protocols of Cupric-Soap Reaction'
 +
},
 +
{
 +
title : 'Krebs-Ringer Phosphate(KRPD Buffer Solution)',
 +
href : '#Krebs-Ringer Phosphate(KRPD Buffer Solution)'
 +
},
 +
                        {
 +
title : 'Cellulose Detection',
 +
href : '#Cellulose Detection'
 +
},
 +
 +
{
 +
title : '<i>In vitro</i> Experiment',
 +
href : '#In vitro Experiment'
 +
},
 +
                        {
 +
title : 'Plate Assay',
 +
href : '#Plate Assay'
 +
},
 +
{
 +
title : 'Materials and Methods of SDS-PAGE',
 +
href : '#Materials and Methods of SDS-PAGE'
}
}
]
]
Line 173: Line 482:
},
},
                                 {
                                 {
-
                                         title : '<strong>Brain-</br>storming</strong>',
+
                                         title : '<strong>Brainstorming</strong>',
                                         dir : []
                                         dir : []
                                 },
                                 },
{
{
title : '<strong>Gallery</strong>',
title : '<strong>Gallery</strong>',
 +
dir : [
 +
    {
 +
title : 'Team',
 +
href : '#team'
 +
},
 +
{
 +
title : 'Presentation',
 +
href : '#presentation'
 +
},
 +
{
 +
title : 'Human Practice',
 +
href : '#human practice'
 +
},
 +
{
 +
title : 'Doodle',
 +
href : '#doodle'
 +
},
 +
{
 +
title : 'Funny Pictures',
 +
href : '#funny pictures'
 +
}]},
 +
                {
 +
title : '<strong>A Letter from Fancy</strong>',
dir : []
dir : []
 +
 +
}
}
],
],
Line 184: Line 518:
{
{
title : '<strong>Outline</strong>',
title : '<strong>Outline</strong>',
-
dir : [
+
                                    dir : []
-
{
+
-
title : 'Introduction',
+
-
href : '#Introduction'
+
-
}
+
-
]
+
},
},
{
{
title : '<strong>Questionaire Survey</strong>',
title : '<strong>Questionaire Survey</strong>',
-
dir : [
+
    dir : [{
-
{
+
title : 'Background',
-
title : 'GMOs and food safety',
+
href : '#Background'
-
href : '#GMOs and food safety'
+
},{
-
}
+
title : 'Methods',
-
]
+
href : '#Methods'
 +
},{
 +
title : 'Results',
 +
href : '#Results'
 +
},{
 +
title : 'Discussion',
 +
href : '#Discussion'
 +
}]
},
},
{
{
title : '<strong>Publicity</strong>',
title : '<strong>Publicity</strong>',
-
dir : [{
+
    dir : []
-
title : 'Introducing iGEM and synthetic biology to the public',
+
-
href : '#Introducing iGEM and synthetic biology to the public'
+
-
}]
+
}
}
-
]
+
] };
-
};
+
//*************************自定义数据部分*************************
//*************************自定义数据部分*************************
</script>
</script>
Line 535: Line 868:
</li>
</li>
<li class="nav-outerLi">
<li class="nav-outerLi">
-
<a href="https://2012.igem.org/Team:WHU-China/Standard"><h2 class="nav-h24" title="Standard"></h2></a>
+
<a href="https://2012.igem.org/Team:WHU-China/Modeling"><h2 class="nav-h24" title="Modeling"></h2></a>
-
<ul class="nav-innerUl nav-innerUl4" name="Standard"></ul>
+
<ul class="nav-innerUl nav-innerUl4" name="Modeling"></ul>
</li>
</li>
<li class="nav-outerLi">
<li class="nav-outerLi">
-
<a href="https://2012.igem.org/Team:WHU-China/Notes"><h2 class="nav-h25" title="Notes"></h2></a>
+
<a href="https://2012.igem.org/Team:WHU-China/Notebooks"><h2 class="nav-h25" title="Notebooks"></h2></a>
-
<ul class="nav-innerUl nav-innerUl5" name="Notes"></ul>
+
<ul class="nav-innerUl nav-innerUl5" name="Notebooks"></ul>
</li>
</li>
<li class="nav-outerLi">
<li class="nav-outerLi">
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<div class="main">
<div class="main">
<div class="passage divcell0">
<div class="passage divcell0">
-
<a name="Introduction"><h3>Introduction</h3></a>
+
<p>  
<p>  
-
<b>GMOs and food safety</b>
+
<h3>GMOs and Food Safety</h3>
</p>
</p>
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</div>
</div>
<div class="passage divcell1">
<div class="passage divcell1">
-
<a name="GMOs and food safety"><h3>GMOs and food safety</h3></a>
+
-
<p><img src="../team/p2.png" /> <b>GMOs and food safety</b>
+
-
</p>
+
-
<p><p align="left">
+
 
-
<b>Background: </b>
+
<a name="Background"><h3>Background</h3></a>
</br>
</br>
-
Genetically modified organisms (GMOs) have become the focus of public attention for several years. Along with the flourish of biotechnology, there are more and more GMOs available for people. However, the disputes around this issue never subside. This year, since our project is strongly associated with GMOs or genetically modified food, we believed that people’s perception on GM food is essential to understand the prospect of modified bacterial product. Therefore, we decided to conduct a cross-sectional study in order to figure out to what extend people would accept consume GMO and bacterial product.  
+
<p align="left">Genetically modified organisms (GMOs) have become the focus of public attention for several years. Along with the flourish of biotechnology, there are more and more GMOs available for people. However, the disputes around this issue never subside. This year, since our project is strongly associated with GMOs or genetically modified food, we believed that people’s perception on GM food is essential to understand the prospect of modified bacterial product. Therefore, we decided to conduct a cross-sectional study in order to figure out to what extend people would accept consume GMO and bacterial product.  
</p>
</p>
-
<p><p align="left">
+
 
-
<b>Methods: </b> </br>
+
<a name="Methods"><h3>Methods</h3></a>
-
We handed out the questionnaires around campus in Wuhan University. Conducted after the enrollment of first-year students, the survey contains data from newly-coming students. We summarize and tabulate data by STATA. P-value was calculated and 95% confidence interval was chosen to see the factors contribute to different opinion on a specific issue.
+
<p align="left">We handed out the questionnaires around campus in Wuhan University. Conducted after the enrollment of first-year students, the survey contains data from newly-coming students. We summarize and tabulate data by STATA. P-value was calculated and 95% confidence interval was chosen to see the factors contribute to different opinion on a specific issue.
</br>
</br>
Prior to the cross-sectional study, we think a pilot study is necessary, because no one in our team has the experience of doing survey in social science either did we get previous data as reference.
Prior to the cross-sectional study, we think a pilot study is necessary, because no one in our team has the experience of doing survey in social science either did we get previous data as reference.
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Change some open questions to close question making it easier for us to count.
Change some open questions to close question making it easier for us to count.
</p>
</p>
-
<p><p align="left">
+
 
-
<b>Results: </b> </br>
+
<a name="Results"><h3>Results</h3></a>
-
As it is shown in figure 1, 2 and 3, the number of participants in different groups was still not evenly distributed, thus we decided to look into those data by grades, sexes and majors. Besides, the general result would be adjusted by different groups.
+
<p align="left">As it is shown in figure 1, 2 and 3, the number of participants in different groups was still not evenly distributed, thus we decided to look into those data by grades, sexes and majors. Besides, the general result would be adjusted by different groups.
</br>
</br>
<br />
<br />
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Grades: 1 for first year, 2 for second year, 3 for third year, 4 for fourth year, 5 for postgraduates Majors: 1 stands for art and history, 2 for social science, 3 for natural science, 4 for engineering, 5 for informatics and technology, 6 for medicine
Grades: 1 for first year, 2 for second year, 3 for third year, 4 for fourth year, 5 for postgraduates Majors: 1 stands for art and history, 2 for social science, 3 for natural science, 4 for engineering, 5 for informatics and technology, 6 for medicine
</p>
</p>
-
<p>
 
<p align="left">
<p align="left">
There were some difference between grades upon attitude toward consumption of bacteria; however, it was not significant enough. No difference between sex, major and consumption of bacteria, respectively. See table 1, 2, 3.
There were some difference between grades upon attitude toward consumption of bacteria; however, it was not significant enough. No difference between sex, major and consumption of bacteria, respectively. See table 1, 2, 3.
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When it comes to the attitude toward GMOs when shopping, complains about the overwhelming GM products in the market emerged in our survey. Most participants from different grade, major, gender agreed that there were so many GM foods in market that we cannot even avoid; but if someone gas got a chance to know it’s genetically modified, they would not buy it. What’s more, we discovered accordance between people’s general idea and people’s attitude when shopping. Most people opposed GMOs doing their best to avoid GMOs, while people holding neutral or positive opinions did not care much about whether they brought a GMO. See table 8 for more details.
When it comes to the attitude toward GMOs when shopping, complains about the overwhelming GM products in the market emerged in our survey. Most participants from different grade, major, gender agreed that there were so many GM foods in market that we cannot even avoid; but if someone gas got a chance to know it’s genetically modified, they would not buy it. What’s more, we discovered accordance between people’s general idea and people’s attitude when shopping. Most people opposed GMOs doing their best to avoid GMOs, while people holding neutral or positive opinions did not care much about whether they brought a GMO. See table 8 for more details.
</br>
</br>
-
<img src="https://static.igem.org/mediawiki/2012/b/bd/Table-8.jpg" width="500" height="100" hspace="2" vspace="1" border="2"  
+
<img src="https://static.igem.org/mediawiki/2012/b/bd/Table-8.jpg" width="500" height="" hspace="2" vspace="1" border="2"  
align="float:left" />
align="float:left" />
<br />
<br />
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As for the knowledge of participants for GMOs, we designed multiple choices to know people’s idea of them except from the benefits of GMOs, such as increasing yield of product, etc. While, some participants realized the issue raised by GMOs, some other people were not very sure about them. See figures for details.
As for the knowledge of participants for GMOs, we designed multiple choices to know people’s idea of them except from the benefits of GMOs, such as increasing yield of product, etc. While, some participants realized the issue raised by GMOs, some other people were not very sure about them. See figures for details.
</br>
</br>
-
<img src="https://static.igem.org/mediawiki/2012/e/ee/Figures.jpg" width="500" height="700" hspace="2" vspace="1" border="2"  
+
<img src="https://static.igem.org/mediawiki/2012/e/ee/Figures.jpg" width="500" height="600" hspace="2" vspace="1" border="2"  
align="float:left" />
align="float:left" />
<br />
<br />
</p>
</p>
-
<p><p align="left">
+
<a name="Discussion"><h3>Discussion</h3></a>
-
<b>Discussion: </b> </br>
+
<p align="left">At the beginning of our survey, we were trying to find a validated questionnaire which would be comparable to our survey in Wuhan University. However, we didn’t find one, so we pre-tested the new questionnaire. Our pilot study revealed lots of problems we did not noticed before. Unfortunately, some problems were hard to control due to limit of time, unfamiliar with doing survey and irregular change of activities in camps. There problems appeared quite obvious on the result, limited participation, mistakes when entering the data and unexpected distribution of students from different background.
-
At the beginning of our survey, we were trying to find a validated questionnaire which would be comparable to our survey in Wuhan University. However, we didn’t find one, so we pre-tested the new questionnaire. Our pilot study revealed lots of problems we did not noticed before. Unfortunately, some problems were hard to control due to limit of time, unfamiliar with doing survey and irregular change of activities in camps. There problems appeared quite obvious on the result, limited participation, mistakes when entering the data and unexpected distribution of students from different background.
+
</br></br>
-
</br>
+
However, most of our results were not impacted by these problems according to our adjusted analysis in different groups (this process only shown when there are impacts). Therefore, our results of people’s perspectives toward GMOs are basically reliable and instructive. So far, we cannot address the question that either grades or major is a confounder for people’s ideas to GMOs due to the shortage of participants in certain major like medicine. Therefore, if given more time, we are able to figure that out.
However, most of our results were not impacted by these problems according to our adjusted analysis in different groups (this process only shown when there are impacts). Therefore, our results of people’s perspectives toward GMOs are basically reliable and instructive. So far, we cannot address the question that either grades or major is a confounder for people’s ideas to GMOs due to the shortage of participants in certain major like medicine. Therefore, if given more time, we are able to figure that out.
-
</br>
+
</br></br>
The results of our survey show that students knew a lot than we had expected. Nevertheless, the uncertainty of GMOs still bothering people, maybe no one can give a right answer that to what extent they are acceptable in our market and on tables of ordinary people. It also provides us an idea that it is possible to counteract GMOs by GMOs. Can we look into the issue raised by genetic engineering via methods we used in synthetic biology? Can we create something to prevent problems caused by GMOs? We hope that more scientists as well as talented students will come with ideas to address our uncertainty about them.
The results of our survey show that students knew a lot than we had expected. Nevertheless, the uncertainty of GMOs still bothering people, maybe no one can give a right answer that to what extent they are acceptable in our market and on tables of ordinary people. It also provides us an idea that it is possible to counteract GMOs by GMOs. Can we look into the issue raised by genetic engineering via methods we used in synthetic biology? Can we create something to prevent problems caused by GMOs? We hope that more scientists as well as talented students will come with ideas to address our uncertainty about them.
</p>
</p>
</div>
</div>
<div class="passage divcell2">
<div class="passage divcell2">
-
<h3>Introducing iGEM and synthetic biology to the public</h3>
+
<p>
<p>
-
<b>Introducing iGEM and synthetic biology to the public<b>
+
<b>Introducing iGEM and synthetic biology to the public</b>
</p>
</p>
Line 723: Line 1,052:
In order to familiarize people, meanly college students with synthetic biology and iGEM, we joined the Wuhan Science Festival, and presented simply modified bacteria to give the public a brief impression on biotechnology.
In order to familiarize people, meanly college students with synthetic biology and iGEM, we joined the Wuhan Science Festival, and presented simply modified bacteria to give the public a brief impression on biotechnology.
<br />
<br />
-
<img src="https://static.igem.org/mediawiki/2012/1/19/Img_9866.jpg" width="500" height="350" hspace="2" vspace="1" border="2" align="float:left" />
+
<img src="https://static.igem.org/mediawiki/igem.org/1/1a/Human_practice9.jpg" width="500" height="350" hspace="2" vspace="1" border="2" align="float:left" />
<br />
<br />
Inserted with RFP, colonies in plates presented a red color that inspired pedestrians and they are curious about why bacteria can emit fluorescence. We explained to them basic molecular biology methods and the difference between molecular biology and synthetic biology, which is aiming at constructing functional organisms based on standardized bio-parts. Our efforts may end to a more correct and truer image of biology, rather than counting birds outdoor and recognizing different plants.
Inserted with RFP, colonies in plates presented a red color that inspired pedestrians and they are curious about why bacteria can emit fluorescence. We explained to them basic molecular biology methods and the difference between molecular biology and synthetic biology, which is aiming at constructing functional organisms based on standardized bio-parts. Our efforts may end to a more correct and truer image of biology, rather than counting birds outdoor and recognizing different plants.

Latest revision as of 03:47, 27 October 2012

    GMOs and Food Safety

    Genetically modified organisms (GMOs) have become the focus of public attention for several years. Along with the flourish of biotechnology, there are more and more GMOs available for people. However, the disputes around this issue never subside. This year, since our project is strongly associated with GMOs or genetically modified food, we believed that people’s perception on GM food is essential to understand the prospect of modified bacterial product. Therefore, we decided to conduct a cross-sectional study in order to figure out to what extend people would accept consume GMO and bacterial product.

    Introducing iGEM and synthetic biology to the public

    In order to familiarize people, meanly college students with synthetic biology and iGEM, we joined the Wuhan Science Festival, and presented simply modified bacteria to give the public a brief impression on biotechnology.

    Background


    Genetically modified organisms (GMOs) have become the focus of public attention for several years. Along with the flourish of biotechnology, there are more and more GMOs available for people. However, the disputes around this issue never subside. This year, since our project is strongly associated with GMOs or genetically modified food, we believed that people’s perception on GM food is essential to understand the prospect of modified bacterial product. Therefore, we decided to conduct a cross-sectional study in order to figure out to what extend people would accept consume GMO and bacterial product.

    Methods

    We handed out the questionnaires around campus in Wuhan University. Conducted after the enrollment of first-year students, the survey contains data from newly-coming students. We summarize and tabulate data by STATA. P-value was calculated and 95% confidence interval was chosen to see the factors contribute to different opinion on a specific issue.
    Prior to the cross-sectional study, we think a pilot study is necessary, because no one in our team has the experience of doing survey in social science either did we get previous data as reference.
    In the questionnaire, we designed two parts to investigate whether people hold negative attitude toward GMO and bacterial product. Considering consuming bacteria seems undesirable to people, we set a example of yogurt at the middle of the questionnaire to see if people can accept eating bacteria after thinking existing product available to everyone today.
    In the pilot study, we handed out 217 questionnaires, and excluded 18 uncompleted ones. In the 199 samples we collected, sex and major were evenly distributed in the library where we handed out the questionnaire. However, students in high grade tended to study at library, while first and second-year students may choose to participate in social activities and face less pressure on entering graduate school.
    Another problem emerged in this pilot is that our questionnaire was a little difficult for students to follow, meanly because of many choice-oriented questions. Many participants gave us advices on doing survey and designing questionnaire which contributed a lot to our final cross-sectional study.
    Before the cross-sectional study, we refined our strategy and questionnaire:
    Change some instructions in questionnaire to arrows at the end of each option, which orient them to the next question they are supposed to answer.
    Change some open questions to close question making it easier for us to count.

    Results

    As it is shown in figure 1, 2 and 3, the number of participants in different groups was still not evenly distributed, thus we decided to look into those data by grades, sexes and majors. Besides, the general result would be adjusted by different groups.


    Figure 1: Distribution of participants by grade

    Figure 2: Distribution of participants by sex

    Figure 3: Distribution of participants by major
    Grades: 1 for first year, 2 for second year, 3 for third year, 4 for fourth year, 5 for postgraduates Majors: 1 stands for art and history, 2 for social science, 3 for natural science, 4 for engineering, 5 for informatics and technology, 6 for medicine

    There were some difference between grades upon attitude toward consumption of bacteria; however, it was not significant enough. No difference between sex, major and consumption of bacteria, respectively. See table 1, 2, 3.

    Table 1: Analysis of variance for grade and consumption of bacteria, P-value>0.05, thus no significant difference

    Table 2: Analysis of variance for sex and consumption of bacteria, P-value>>0.05.

    Table 3: Analysis of variance for major and consumption of bacteria, P-value>>0.05

    Since there was no significant difference between groups, we could conclude that the general result for people’s willingness to eat bacteria is: see table 4.

    Table 4: 1 stands for Yes, 2 stands for No, 3 stands for it depends, 6 was a typing error.

    We wanted to know whether people would change their mind if they were told that bacteria are normal in our surrounding and even on our table. For that purpose, we designed a leading question to remind participants that yogurt contains bacteria. The result shown that 26/73 students who chose NO changed their minds to YES after showing them the example of yogurt. 17/73 participants changed from NO to IT DEPENDS.

    For people’s idea of GMOs, we measured general attitude toward GMOs, choice of food when shopping, and the knowledge about GMOs. There are differences between grades, result shown in table 5 and figure 4. High proportion of postgraduate students honestly chose “don’t know”, which shown by “0”. Besides, the proportion of postgraduates who thought GMOs were “very harmful” (shown by “1”) is higher than any other students. Many first-year students choose “9” which stands for very beneficial.

    Table 5: Analysis of variance for attitude toward GMOs and grade

    Figure 4: Distribution of score given by participants from different grades

    However, we have to exclude grade from potential confounders before drawing the conclusion that grade is a factor for people to have difference ideas. According to the data analysis, seen table 6, students from different major were not evenly distributed in grades.

    Table 6: Distribution of students by majors and grades

    For students from various majors, there were difference between them, seen result at Table 7 and Figure 5.

    Table 7: Analysis of variance for general attitude toward GMOs and major



    Figure 5: Distribution of scores rated by participants from difference major.

    For the relation between attitudes and sex, there was no association discovered.

    When it comes to the attitude toward GMOs when shopping, complains about the overwhelming GM products in the market emerged in our survey. Most participants from different grade, major, gender agreed that there were so many GM foods in market that we cannot even avoid; but if someone gas got a chance to know it’s genetically modified, they would not buy it. What’s more, we discovered accordance between people’s general idea and people’s attitude when shopping. Most people opposed GMOs doing their best to avoid GMOs, while people holding neutral or positive opinions did not care much about whether they brought a GMO. See table 8 for more details.

    Table 8: Distribution of people holding ideas when shopping and their general opinions

    As for the knowledge of participants for GMOs, we designed multiple choices to know people’s idea of them except from the benefits of GMOs, such as increasing yield of product, etc. While, some participants realized the issue raised by GMOs, some other people were not very sure about them. See figures for details.

    Discussion

    At the beginning of our survey, we were trying to find a validated questionnaire which would be comparable to our survey in Wuhan University. However, we didn’t find one, so we pre-tested the new questionnaire. Our pilot study revealed lots of problems we did not noticed before. Unfortunately, some problems were hard to control due to limit of time, unfamiliar with doing survey and irregular change of activities in camps. There problems appeared quite obvious on the result, limited participation, mistakes when entering the data and unexpected distribution of students from different background.

    However, most of our results were not impacted by these problems according to our adjusted analysis in different groups (this process only shown when there are impacts). Therefore, our results of people’s perspectives toward GMOs are basically reliable and instructive. So far, we cannot address the question that either grades or major is a confounder for people’s ideas to GMOs due to the shortage of participants in certain major like medicine. Therefore, if given more time, we are able to figure that out.

    The results of our survey show that students knew a lot than we had expected. Nevertheless, the uncertainty of GMOs still bothering people, maybe no one can give a right answer that to what extent they are acceptable in our market and on tables of ordinary people. It also provides us an idea that it is possible to counteract GMOs by GMOs. Can we look into the issue raised by genetic engineering via methods we used in synthetic biology? Can we create something to prevent problems caused by GMOs? We hope that more scientists as well as talented students will come with ideas to address our uncertainty about them.

    Introducing iGEM and synthetic biology to the public

    In order to familiarize people, meanly college students with synthetic biology and iGEM, we joined the Wuhan Science Festival, and presented simply modified bacteria to give the public a brief impression on biotechnology.

    Inserted with RFP, colonies in plates presented a red color that inspired pedestrians and they are curious about why bacteria can emit fluorescence. We explained to them basic molecular biology methods and the difference between molecular biology and synthetic biology, which is aiming at constructing functional organisms based on standardized bio-parts. Our efforts may end to a more correct and truer image of biology, rather than counting birds outdoor and recognizing different plants.



    Besides, we introduced to students about our project this year. Induced by an epic background, the characters of our project were three bacteria fighting against fatty acids, glucose and death. Based on the story, we talked with students about the purpose and how we can achieve the goal. The public in Wuhan were not very familiar with biotechnology and the use of biotechnology. Apart from what we did in lab, going out to meet people is as the same importance as results under UV light in iGEM.

    As for iGEM, we introduced to audience this competition regarding its history, its goals and participation in China. As a top-ranking university in China, we think that students of Wuhan University are supposed to know more about academic events. It not only smoothes our project but also encourages more students with ideas to participate in international competition. Furthermore, we hope that more students in Wuhan would pay more attention to competitions in biology as well as College of Life Science of Wuhan University. We believe our effort will benefit subsequent candidates in the long run, and help them get more funding easier.