Team:Tianjin/Project

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2012年7月21日，第十一届中国国际合唱节圆满落下帷幕，天津大学北洋合唱团凭借原创作品《三十幅》和拉脱维亚作品《祈求》两首合唱曲目，在166支国内外优秀的合唱团中脱颖而出，一举夺得了成人混声组金奖，优秀钢琴伴奏奖第一名的好成绩，在北洋的众多荣誉中又增添了浓墨重彩的一笔。
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中国国际合唱节创办于1992年，今年时逢20周年，本届国际合唱节报名团体达166支，境外团体32支，其中有九支全球顶级的合唱团，评委、团员来自五大洲39个国家和地区；国内团体134支，来自23个省、市、自治区，总参赛人数近万人。这是自1992年该节创办以来规模最大、人数最多的一届，天津大学北洋合唱团受邀参加这次的比赛，也充分体现了合唱团在合唱界的水准和影响力。
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北洋合唱团十分重视这次比赛，无论是指挥还是团员们，在得知即将参加这次国际合唱节后，都放弃了假期，每天分为上午和下午两个单元进行集训，晚上也经常加班加点的排练，希望有一个饱满的精神状态，并在做好充分准备的情况下参加比赛，在每一个音符上每一个力度上都仔细揣摩，认真分析，力求将一中一洋两首作品完美演绎。
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在经过了半个多月紧张的排练后，北洋合唱团迎来了自己的比赛。2012年7月17日中午，合唱团抵达北京，在稍作休整后，团长便将大家集合起来进行比赛前的最后一次发声练习和曲目排练，北洋合唱团常任指挥任宝平老师也一直鼓励大家，并让大家放松心态，最后一次强调曲目中需要注意的地方。下午5点，合唱团到达中央民族音乐厅，在组委会的协助下进行走台和彩排。晚上7点，比赛正式开始，天津大学北洋合唱团作为当天成人混声D组的第一支队伍，用一首北洋原创的曲目《三十幅》拉开了比赛的序幕。
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《三十幅》由北洋合唱团驻团作曲家崔薇老师创作，歌词选自道家创始人老子的《道德经》，道家的“无”的思想和精神在这首作品中得到了很好的阐释，歌词与旋律相得益彰，因此在演唱时需要对歌词有深刻的理解，技巧上和音准上的要求也很高，只有这种高要求才能将这首作品中所蕴含的中国传统思想中内在的东西表现出来。北洋在这点上做的很到位，在台上将这首作品演绎的淋漓尽致，开场就博得了满堂喝彩。
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接下来是一首拉脱维亚作品《祈求》，这部作品是根据格列高素歌的元素所创作的现代的、高难度的合唱作品，有很大的演唱难度。整首作品分为三部分，每一部分的难度都很高,在排练的过程中遇到了很多的困难,包括和乐器的配合上,力度的协调上等等,因此在演唱时对作品的把握很困难,平时的排练也着重的强调了这些问题,每一位团员都很敬业,不断地揣摩,纠正发音,比赛当天,合唱团发挥出了正常的水平,平时一次次强调的问题在舞台上都没有出现,大家的努力最终没有白费.这首作品演绎的很完美.
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正是由于勤勤恳恳的排练和比赛时正常的发挥,天津大学北洋合唱团在166支国内外优秀的团队中夺得了成人混声组的金奖,并且获得了优秀钢琴伴奏奖的第一名，合唱团出色的表现赢得了国内外评委的高度赞扬，很多国际知名的评委在看到北洋的演出后都表示，“北洋音色”是走在世界的最前端的，北洋合唱团是这次比赛中他们所看到的最出色的合唱团。
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在即将到来的2012韩国丽水世博会中,北洋合唱团将代表中国赴韩国参加演出,期待他们更加精彩的表现..
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<p class="menu_head">Project Contents</p>
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<a href="https://2012.igem.org/Team:Tianjin/Project/OrthogonalSystem">Orthogonal System</a>
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        </div>
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<a href="https://2012.igem.org/Team:Tianjin/Project/Regulation">Logic Metabolism Regulation</a>
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<a href="https://2012.igem.org/Team:Tianjin/Project/Gene">Future Work</a>
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<a href="https://2012.igem.org/Team:Tianjin/Data">BioBrick</a>
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<a href="https://2012.igem.org/Team:Tianjin/Project/Technology">Technology</a>
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<p class="menu_head">In this page</p>
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<a href="#Orthogonal_System">Orthogonal System</a>
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<a href="#Gene_Pollution_Prevention_and_Gene_Encryption">Safety Encryption</a>
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<a href="#Logic_Metabolism_Regulation">Logic Metabolism Regulation</a>
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<center><span style="font-size:46px;font-family:Cambria;margin-top:10px;line-height:80%">Project</span></center>
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<br>
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The Glossary:
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* The O-Key System -- Any orthogonal system containing a pair of orthogonal ribosome and mRNA
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* The O-Key -- the orthogonal ribosome, which serves like a key to translate the orthogonal mRNA
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* The O-Lock -- the orthogonal mRNA, which can only be deciphered by the O-Key.
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=Orthogonal System=
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[[file:TJU2012-Proj-fig-1.png|thumb|200px|right|'''Figure 1.''' Word "RNA" (from "http://www.mfpl.ac.at")]]
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By rationally mutate the Shine-Dalgarno (SD) and anti-Shine-Dalgarno (ASD) sequence, we are able to take advantage of the interaction of mRNA and ribosome to build our O-Key System of orthogonal ribosome and orthogonal mRNA. Within this system, we constructed an operon containing RFP and GFP coding sequence to verify the orthogonality of the O-Key. By selectively mutate the SD sequence of RFP or GFP, we were able to establish four translation pathways to characterize the effect of the O-Key System. In addition, we set up a model to predict the output of GFP and RFP under various circumstances. The model calculates the ΔG of ASD and SD sequence binding, and make use of this energy to evaluate the feasibility and translation efficiency of our O-Key System. The model turned out to be highly convincing as it corresponds with our wet lab result. In the end, both the wet and dry lab results matches our design. 
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=<span style="line-height:100%">Genetic Pollution Prevention and Genetic Encryption</span>=
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[[file:TJU2012-Proj-fig-2.png|thumb|400px|right|'''Figure 2.''' Comic of genetic pollution defence (from TJU iGEM　Team 2012)]]Aiming at preventing genetic pollution, we employed the O-Key System to establish a translational fence that can restrain unwanted protein expression. The convenience and effectiveness of the O-Key System will make it applied to a larger scale in genetic engineering. We predict different companies will embed the O-Key system in their various product to ensure biosafety. In the meantime, because the O-Key System includes a key and a lock, we can make use of this mechanism to encrypt information into cell or locking the product information. This characteristic showed a promising application in information encryption, intellectual property protection, etc. Furthermore, the O-Key System can be applied to the entire organism to construct an orthogonal organism. We began with the simplest creature - the phage, and worked on the RBS of its various protein. After mutation, the phage becomes a brand new orthogonal organism that can only infect the cells with orthogonal ribosomes.  Using this O-Key Phage, we greatly reduce risk of phage pollution in the lab, while performing regular experiment using the phage. At last, a successful interdisciplinary model that combines marketing and bioengineering was constructed to predict the diffusion of exogenous gene across space and time. This creative model used the analogy of human society and bacteria colony to predict the speed and probability of genetic transfer.
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=Logic Metabolism Regulation=
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[[file:TJU2012-Proj-fig-3.png|thumb|300px|right|'''Figure 3.''' Metabolism Network (from TJU iGEM　Team 2012)]]
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In this section, we describe the principles of Yeast Assembler, a novel way of assemble multiple fragments into a long operon, and specifically used this method to construct the gene needed to produce Violacein. The pathway of expressing violacein consists of five genes, and they build up a long operon. The conventional assembly methods for violacein takes too much time and labor, up to several weeks and offer resulting in failure, but using Yeast Assembler we can complete the whole process in a week. We will introduce and elaborate on the assembler in details. Through such an experiment, we could also prove the feasibilities of the O-Key System in regulating metabolism. Furthermore, we talked about the application of AND gate based on O-Key System in adjusting metabolism.
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Latest revision as of 16:00, 26 October 2012


Project


The Glossary:

  • The O-Key System -- Any orthogonal system containing a pair of orthogonal ribosome and mRNA
  • The O-Key -- the orthogonal ribosome, which serves like a key to translate the orthogonal mRNA
  • The O-Lock -- the orthogonal mRNA, which can only be deciphered by the O-Key.

Orthogonal System

Figure 1. Word "RNA" (from "http://www.mfpl.ac.at")

By rationally mutate the Shine-Dalgarno (SD) and anti-Shine-Dalgarno (ASD) sequence, we are able to take advantage of the interaction of mRNA and ribosome to build our O-Key System of orthogonal ribosome and orthogonal mRNA. Within this system, we constructed an operon containing RFP and GFP coding sequence to verify the orthogonality of the O-Key. By selectively mutate the SD sequence of RFP or GFP, we were able to establish four translation pathways to characterize the effect of the O-Key System. In addition, we set up a model to predict the output of GFP and RFP under various circumstances. The model calculates the ΔG of ASD and SD sequence binding, and make use of this energy to evaluate the feasibility and translation efficiency of our O-Key System. The model turned out to be highly convincing as it corresponds with our wet lab result. In the end, both the wet and dry lab results matches our design.

Genetic Pollution Prevention and Genetic Encryption

Figure 2. Comic of genetic pollution defence (from TJU iGEM　Team 2012)
Aiming at preventing genetic pollution, we employed the O-Key System to establish a translational fence that can restrain unwanted protein expression. The convenience and effectiveness of the O-Key System will make it applied to a larger scale in genetic engineering. We predict different companies will embed the O-Key system in their various product to ensure biosafety. In the meantime, because the O-Key System includes a key and a lock, we can make use of this mechanism to encrypt information into cell or locking the product information. This characteristic showed a promising application in information encryption, intellectual property protection, etc. Furthermore, the O-Key System can be applied to the entire organism to construct an orthogonal organism. We began with the simplest creature - the phage, and worked on the RBS of its various protein. After mutation, the phage becomes a brand new orthogonal organism that can only infect the cells with orthogonal ribosomes. Using this O-Key Phage, we greatly reduce risk of phage pollution in the lab, while performing regular experiment using the phage. At last, a successful interdisciplinary model that combines marketing and bioengineering was constructed to predict the diffusion of exogenous gene across space and time. This creative model used the analogy of human society and bacteria colony to predict the speed and probability of genetic transfer.

Logic Metabolism Regulation

Figure 3. Metabolism Network (from TJU iGEM　Team 2012)

In this section, we describe the principles of Yeast Assembler, a novel way of assemble multiple fragments into a long operon, and specifically used this method to construct the gene needed to produce Violacein. The pathway of expressing violacein consists of five genes, and they build up a long operon. The conventional assembly methods for violacein takes too much time and labor, up to several weeks and offer resulting in failure, but using Yeast Assembler we can complete the whole process in a week. We will introduce and elaborate on the assembler in details. Through such an experiment, we could also prove the feasibilities of the O-Key System in regulating metabolism. Furthermore, we talked about the application of AND gate based on O-Key System in adjusting metabolism.

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