Team:LMU-Munich/Project

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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== '''Overall project''' ==
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[[File:Project draft.png|500px|right]]
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The goal of our project BEADzillus is the production of ''Bacillus subtilis'' spores which display different proteins with special features on their surface.  
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For example, the spore could:
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<a href="https://2012.igem.org/Team:LMU-Munich/Project" style="left: 276px; top: 40px; width: 271px; height:265px;"><img style="left: -276px; top: -40px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home0.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins" style="left: 130px; top: 50px; width: 146px; height:145px;"><img style="left: -130px; top: -50px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home1.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop" style="left: 166px; top: 196px; width: 110px; height:110px;"><img style="left: -166px; top: -196px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home2.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks" style="left: 55px; top: 160px; width: 110px; height:110px;"><img style="left: -55px; top: -160px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home3.png"></a>
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- bind harmful viruses
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<p align=justify>
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Our project '''Bead'''zillus is composed of several modules. Click on the symbols to learn more about their function.
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- bind toxic metals
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The idea of '''Bead'''zillus is to enrich the [http://partsregistry.org/Main_Page partsregistry] with new BioBricks that are needed for the work with ''B. subtilis'' and show how its unique features lead to new creative ideas. We use the spores of ''B. subitlis'' to establish a platform for protein display.
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- bind plastic molecules
 
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- expose enzymes
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*[[File:Bacilluss_Intro.png|30px|link=Team:LMU-Munich/Bacillus_Introduction]] [[Team:LMU-Munich/Bacillus_Introduction|'''''Bacillus'' Intro''']]: A short introduction to the organism ''Bacillus subtilis'' and why we chose to work with it.
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*[[File:BacillusBioBrickBox.png|30px|link=Team:LMU-Munich/Bacillus_BioBricks]] Our [[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]] offers a set of new parts for the work with ''B. subtilis''.
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*[[File:SporeCoat.png|30px|link=Team:LMU-Munich/Spore_Coat_Proteins]] [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]] are a platform to display proteins on ''B. subtilis'' spores.
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*[[File:GerminationSTOP.png|25px|link=Team:LMU-Munich/Germination_Stop]] [[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]] prevents the spores from germinating to vegetative cells.
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to filter fluids  or for use in laboratory.
 
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As a safety issue we will stop spores from outgrowing. First we want to knock out several genes which are important for germination and second we want potentially outgrowing spores to be killed by a toxin they produce themselves.
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*On our [[Team:LMU-Munich/Data|Data]] page you find all our results in detail.
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== Project Details==
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*Why did we chose spores to display proteins and for what could they be used? See our [[Team:LMU-Munich/Application|Applications page]].
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[[File:Project arrow 1.png|500px|center]]
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Visit the other pages related to our project:
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* [[Team:LMU-Munich/Attributions|Attributions]] who helped us.
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* [[Team:LMU-Munich/Collaborations|Collaborations]] with other iGEM Teams.
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* [[Team:LMU-Munich/Parts|Registry]] Here is the list of all new BioBricks we've added to the registry so far.
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* [[Team:LMU-Munich/Strains|''Bacillus'' strains]] Here you find information about the ''B. subtilis'' strains we created and how to obtain them.
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* [[Team:LMU-Munich/Inverter|Inverter]] See here a special project we continued from the LMU-Munich 2011 Team.
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* [[Team:LMU-Munich/Achievements|Achievements]] Find here which medal we would give ourselves and why.
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=== Clone Vectors ===
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<div class="box">
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====Project Navigation====
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Part of establishing ''Bacillus subtilis'' to the BioBrick mode is to modify standard vectors of Bacillus.
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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=== Evaluate Promoters ===
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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Another point is to produce promoters of Bacillus subtilis in BioBrick mode and to evaluate them.  
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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|}
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=== Fuse Spore Coat Proteins ===
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=== Stop Germination ===
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== Results ==
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{{:Team:LMU-Munich/Templates/Page Footer}}
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Latest revision as of 12:25, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU red and white.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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