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Team:Berkeley/Project
From 2012.igem.org
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When we engineer systems in synthetic biology using cellular features such as motility, morphology and subcellular localization, we usually like to do so using libraries. | When we engineer systems in synthetic biology using cellular features such as motility, morphology and subcellular localization, we usually like to do so using libraries. | ||
| - | This is due to our current limitation of guessing which | + | This is due to our current limitation of guessing which parameters to choose to optimize a given system. By using libraries, we can examine a large parameter space and find trends present in our system and even optimal parameters to use for our design. |
Why do we want to use libraries? --Harneet | Why do we want to use libraries? --Harneet | ||
Revision as of 08:49, 3 October 2012
MiCodes: Enabling library screens with microscopy by connecting genotypes to observable phenotypes
Many applications in synthetic biology demand precise control over subcellular localization, cell morphology, motility, and other such phenotypes that are only observable via microscopy. At present, engineering these properties is challenging due in large part to the inherent throughput limitation imposed by microscopy. We have developed a strategy that enables high-throughput library screening with microscopy by coupling a unique fluorescence signature with each genotype present in a library. These MiCodes (microscopy barcodes) are generated by targeting combinations of fluorophores to several organelles within yeast, and they eliminate the need to isolate and observe clonal populations separately. MiCodes can potentially scale to library sizes of 10^6 or more, and their analysis can be largely automated using existing image processing software. As a proof of principle, we applied MiCodes to the problem of finding unique pairs of protein-protein interaction parts.
Why do we want to use microscopy? --Harneet.
When we engineer systems in synthetic biology using cellular features such as motility, morphology and subcellular localization, we usually like to do so using libraries. This is due to our current limitation of guessing which parameters to choose to optimize a given system. By using libraries, we can examine a large parameter space and find trends present in our system and even optimal parameters to use for our design. Why do we want to use libraries? --Harneet
