Team:NRP-UEA-Norwich/Week0
From 2012.igem.org
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==Project Planning (05/12 - 06/12)== | ==Project Planning (05/12 - 06/12)== | ||
- | Closer to the start date, we decided we wanted to build a project based around the expertise of UEA and the NRP and learn from our predecessors. We realised that a plant project would not realistically fit within a ten week project if any mistakes | + | Closer to the start date, we decided we wanted to build a project based around the expertise of UEA and the NRP and learn from our predecessors. We realised that a plant project would not realistically fit within a ten week project if any mistakes were made or major challenges faced. After much consideration, we decided upon a project on NO (Nitric Oxide) sensing within a bacterial chassis. |
We thought NO was particularly interesting due to the wide number of environments it is involved in and hence a NO sensor could be widely applied. Within these weeks we were fortunate to have a number of speakers, who were involved in the research of NO. These talks gave us a better understanding of some of the challenges surrounding the sensing of NO. Among these problems, we wanted to improve the flexibility of the sensor and also the specificity. | We thought NO was particularly interesting due to the wide number of environments it is involved in and hence a NO sensor could be widely applied. Within these weeks we were fortunate to have a number of speakers, who were involved in the research of NO. These talks gave us a better understanding of some of the challenges surrounding the sensing of NO. Among these problems, we wanted to improve the flexibility of the sensor and also the specificity. | ||
- | Tackling one problem at a time, we designed a hybrid promoter combining a CArG element from mammalian cells and the PyeaR from bacterial cells. As we did not know how the individual promoters would affect the cell, we designed the hybrid promoter to have BamH1 between the two promoters. This would allow us to restrict and separate the two. In addition to this, we sent off the hybrid promoter for synthesis in two orientations; one where the bacterial PyeaR is upstream of CArG and the other reversed. | + | Tackling one problem at a time, we designed a hybrid promoter combining a CArG element from mammalian cells and the PyeaR from bacterial cells. As we did not know how the individual promoters would affect the cell, we designed the hybrid promoter to have BamH1 between the two promoters. This would allow us to restrict and separate the two. In addition to this, we sent off the hybrid promoter for synthesis in two orientations; one where the bacterial PyeaR is upstream of CArG and the other reversed. |
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==Human Practice Planning (06/12-07/12)== | ==Human Practice Planning (06/12-07/12)== | ||
With genes sent off for synthesis and no lab space yet, we turned our minds to the criteria for the medals. As most of the criteria were aimed at work on BioBricks, we looked at the human practice element of the gold medal. We decided upon the creation of a film and to do this, we wanted to collaborate with an artist who had shown interest in iGEM and contacted us. Within these few weeks we also put together a proposal for grants from the Biochemical society to fund for the artist and also the Enterprise Fund to fund for lab consumables and travel expenses. We later found that we were successful. | With genes sent off for synthesis and no lab space yet, we turned our minds to the criteria for the medals. As most of the criteria were aimed at work on BioBricks, we looked at the human practice element of the gold medal. We decided upon the creation of a film and to do this, we wanted to collaborate with an artist who had shown interest in iGEM and contacted us. Within these few weeks we also put together a proposal for grants from the Biochemical society to fund for the artist and also the Enterprise Fund to fund for lab consumables and travel expenses. We later found that we were successful. |
Revision as of 22:38, 26 September 2012