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Team:Wageningen UR/Parts
From 2012.igem.org
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'''Faulty parts*''' | '''Faulty parts*''' | ||
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| - | After some growth experiments, it was concluded that these biobricks were not functioning as they were expected to. They were then sent for sequencing. | + | After some growth experiments, it was concluded that these biobricks were not functioning as they were expected to. They were then sent for sequencing. It turned out these biobricks lack the iron inducable promotor, meaning they are useless for our project. |
Revision as of 11:52, 25 September 2012
Parts we made
<groupparts>iGEM012 Wageningen_UR</groupparts>
Parts we used
For our project, we used few bricks from the 2012 Spring Distribution Kit. A list is included below.
| Part | Name | Description |
| [http://partsregistry.org/Part:BBa_B0015 Bba_B0015] | Terminator | double terminator (B0010-B0012) |
| [http://partsregistry.org/Part:BBa_I13522 Bba_I13522] | pTet GFP | Untagged GFP behind a constitutive promoter. |
| [http://partsregistry.org/Part:BBa_J04450 Bba_J04450] | RFP Coding Device | RFP Coding Device (iptg + Cap induced, pSB1C3 backbone) |
| [http://partsregistry.org/Part:BBa_J04500 Bba_J04500] | IPTG inducable promotor with RBS | R0010.B0034 |
| [http://partsregistry.org/Part:BBa_K197038 Bba_K197038] | BBb - BseRI/AcuI entry vector | This part is and entry vector in BBb Format. It is flanked by BamHI, BseRI, BglII, and AcuI sites and is used in the 2AB Layered Assembly scheme developed at UC Berkeley. |
Parts we ordered
For our project, we ordered few bricks from the iGEM Registry. A list is included below.
| Part | Name | Description |
| [http://partsregistry.org/Part:BBa_K197021 Bba_K197021] | EILD | One peptide of a heterodimeric leucine zipper that dimerizes with KILR. |
| [http://partsregistry.org/Part:BBa_K197022 Bba_K197022] | KILR | A heterodimeric peptide that dimerizes to EILD. This can be used for adhering two cells together to evolve new two cell chemistry and functions. |
| [http://partsregistry.org/Part:BBa_J3901 Bba_J3901]* | PrFe-mRFP1 | This device coupled the promoters PI and PII of rus operon from Acidithiobacillus ferrooxidans with a monomeric red fluorescent protein (BBa_E1010) used as a signal, showing specific sensorbility response to iron ions presence. |
| [http://partsregistry.org/Part:BBa_I765010 Bba_I765010]* | Iron promoter -EYFP reporter | Iron promoter expresion is reported by the trascription of EYFP reporter. |
Faulty parts*
After some growth experiments, it was concluded that these biobricks were not functioning as they were expected to. They were then sent for sequencing. It turned out these biobricks lack the iron inducable promotor, meaning they are useless for our project.
