LOCUS       pSB1A3            4157 bp ds-DNA     circular     26-SEP-2012
ACCESSION   unknown
VERSION     unknown
KEYWORDS    .
FEATURES             Location/Qualifiers
     gene            complement(1020..1879)
                     /label="Amp"
     misc_feature    2127..2134
                     /label="Not I "
     promoter        2298..2351
                     /label="BBa_R0040"
     RBS             2382..2405
                     /label="BBa_B0030"
     gene            2414..3083
                     /label="BBa_E1010 RFP"
     misc_feature    3160..3165
                     /label="Spe I "
     RBS             3255..3278
                     /label="BBa_B0030"
     gene            3282..3992
                     /label="BBa_E0040 GFP"
     misc_feature    4152..4157
                     /label="Afl II"
ORIGIN      
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1741 ataacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1981 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2041 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2101 ggcggccgcg atgatttctg gaattcgcgg ccgccttaag taagtaagag tatacgtata
     2161 tcggctaaaa cgtattaagg cgcttcggcg ccttttttta tgggggtatt ttcatcccaa
     2221 tccacacgtc caacgcacag caaacaccac gtcgacccta tcagctgcgt gctttctatg
     2281 agtcgttgct gcatatctcc ctatcagtga tagagattga catccctatc agtgatagag
     2341 atactgagca catcagcagg acgcactgac ctttggaatt cattaaagag gagaaaggta
     2401 ccatggcgag tagcgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
     2461 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
     2521 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
     2581 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
     2641 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
     2701 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
     2761 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
     2821 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
     2881 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
     2941 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
     3001 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
     3061 gtcactccac cggtgcttaa taatctgctg agtgatagac tcaaggtcgc tcctagcgag
     3121 tggcctttat gattatcact ttacttatgt gatctgcaga ctagttctag aggcggactc
     3181 actatagagg gacaaactca aggtcattcg caagagtggc ctttatgatt gaccttcttc
     3241 cggttaatca attcattaaa gaggagaaag gtaccatgag caaaggagaa gaacttttca
     3301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     3361 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
     3421 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
     3481 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
     3541 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
     3601 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
     3661 attttaaaga agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca
     3721 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
     3781 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
     3841 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
     3901 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
     3961 ttacacatgg catggatgag ctctacaaat aaggatcggt tgtcgagtaa ggatctccag
     4021 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     4081 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     4141 ttataggatc ccttaag    
//

