"

From 2012.igem.org

I. Inoculate Liquid Cultures for Cultures Which Failed to Grow

• Inoculate one isolated colony (or as close as possible) into ~2 mL of appropriate media according to the following table in sterile culture tubes

Strain	Media
E. coli ECNR1	LB
E. coli SIMD43	LB

• Incubate cultures overnight with shaking at 37 ^oC

• As of now, cultures still have not grown. ~ Srk3 14:10, 26 June 2012 (EDT)