"

From 2012.igem.org

Make plates of pBAV1K-containing DH5α glycerol stocks

• Inoculate a sample of frozen DH5α cells containing the pBAV1K-T5-gfp plasmid onto LB-Kan plates of 10 μg/mL and 50 μg/mL
• Incubate at 34^o C overnight

Make Kan plates of different concentrations (30 and 40 μg/mL)

• Prepare five plates each of LB-Kan plates at concentrations of 30 and 40 μg/mL as above

Prepare O/N cultures of ADP1 glycerol stocks

• Scrape the top of the ADP1 WT glycerol stock and inoculate 2 mL each of LB and SOB
• Incubate at 34^oC overnight for 16 hours