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From 2012.igem.org

Make 15% glycerol stocks (3 total) of successful O/N culture of Bs strain 1A833

• Transfer 600 uL of each liquid culture to each microcentrifuge tube (x3)
• Add 200 uL of 60% glycerol to each tube
• Vortex to mix thoroughly
• Store tubes in the -80^o C freezer
• Discard any remaining liquid culture