Week 12 Resurrection

September 03

Pick up single colonies from plate of TA-9-2 and amplify them in LB liquid medium. Do colony PCR to identify the ligation procedure.

Tuesday, September 06, 2012

1. LIU Xiao continues his mutation D0 library but finds that colonies on plates strange. We suspect antibiotics on these plates are getting worse.

Wednesday, September 06, 2012

2. LIU Xiao made 6 half-finished( one arm long and the other short of D0 MS2 aptamer) and sent sequencing.

Thursday, September 06, 2012

1. Dr. Song from Tianjin University visits us and raises morale in us. She advises us to show off our confidence and strives for modeling things.

2. We restart our lab meeting and plan for last month’s sprint.

September 07, 2012

Gap day……

September 08, 2012

Part Riboscaffold version 1’s sequencing results are so wrong %>_<%, so Yan makes part again.

LIU Xiao continues his mutation D0 experiments.

September 09, 2012

1. Chen and LIU Huachun make competent cells of BL21*DE3.

2. We restart cotransformation and coexpression of RNA scaffold+FA+FB.