Team:WashU/mobile/Project/protocols

From 2012.igem.org

Protocols

General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

Large Scale Double Digest

General Ligation Protocol

General Transformation Protocol

General Gel Purification Protocol

LB Plates (*includes instruction for liquid media)

Glycerol Stocks

SDS-PAGE gel electrophoresis

Coomassie Staining of Acrylamide gels

Cell Lysis and Protein Purification Prep (CALEB)

Synechocystis sp. PCC6803 Protocols

Transformation Synechocystis 6803

Synechocystis 6803 media

Carotenoid Measurement Protocols

Carotenoid Extraction Using Ethanol