Team:UTP-Software/SoftwareTool
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Project DetailsWhat is the S2MT tool?
The SynBio Sequence Mutation Tool (S2MT) it's a MATLAB implementation software that helps teams to start their projects once they have selected some possible BioBricks or DNA sequences to work with. This tool was developed in MATLAB basically for the reason that, what new iGEM teams require is a flexible environment capable of not only running the algorithm that we develop, but also a place where they can continue working their ideas, examine more in detail their sequences and even continue the development of our tool. So we thing that our system will also knock down the barriers that exist between computation and biology and give students the tools required to do better simulations, and save a lot of valuable time! Behind the code there are three simple steps in which the program consists: Flow diagram for the S2MT - Primer Design SectionProtocol Followed to design the primers:[1]
Tm = 81.5 + 0.41(%GC) − 675 / N - where N is the primer length in base pairs. Next S2MT VersionAfter finishing the first version, we had in mind several things to implement to the next:
References[1]. Stratagene. QuikChange™ Site-Directed Mutagenesis Kit. Catalog #200518. Revision #108005h. Available in: http://web.physics.ucsb.edu/~deborah/pro/pro_pdf/Stratagene%20QuikChange.pdf. [2]. Prather:Gibson CBA. Available in: http://openwetware.org/wiki/Prather:Gibson_CBA |