Team:Tokyo Tech/Projects/PHAs/detail/index.htm

From 2012.igem.org

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Production trial of PHAs by past teams

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We introduce some attempts in the past iGEM to show how great our work is in iGEM.

iGEM10_Caltech

Part number Description States experience
BBa_K338003PHA Synthase Composite, Part 1/2Planning
BBa_K338004PHA Synthase Composite, Part 1/2AvailableNone

They couldn’t prove that their engineered bacteria produced PHB according to the team wiki.


iGEM10_INSA-Lyon

Part number Description States experience
BBa_K342001Pha C (poly-β-hydroxybutyrate polymerase)AvailableNone

They registered and submitted a part, which contains only phaC sequence, as worked. But the team wiki shows that the part doesn’t meet the standard which is required in iGEM because they couldn’t cut their part with XbaI.

iGEM09_Duke

Part number Description States experience
BBa_K282000phaABAvailableNone

iGEM08_Utah State

Part number Description States experience
BBa_K089001phaA genePlanning
BBa_K089002phaB genePlanning
BBa_K089003phaC genePlanning

iGEM08_Hawaii

Part number Description States experience
BBa_K125801RBS-phaAAvailableNone
BBa_K125802 RBS-phaBPlanning
BBa_K125803RBS-phaCAvailableNone
BBa_K125804RBS-phaEAvailableNone
BBa_K125501phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)AvailableNone
BBa_K125502phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)AvailableNone
BBa_K125503phaC BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)AvailableNone
BBa_K125504phaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)AvailableNone

iGEM08_Virginia

Part number Description States experience
BBa_K156031RBS + phaA + double terminatorAvailableNone
BBa_K156033RBS + phaB1 + double terminatorAvailableNone
BBa_K156034RBS + phaC1 + double terminatorAvailableNone
BBa_K156012 phaA (acetyl-CoA acetyltransferase)AvailableNone
BBa_K156013phaB1 (acetyacetyl-CoA reductase)AvailableNone
BBa_K156014phaC1 (Poly(3-hydroxybutyrate) polymerase)AvailableNone
BBa_K156021Promoter + RBS + phaA + double terminatorAvailableNone
BBa_K156018Promoter + RBS + phaAPlanning
BBa_K156019Promoter + RBS + phaB1Planning
BBa_K156020Promoter + RBS + phaC1Planning
BBa_K156022 Promoter + RBS + phaB1 + double terminatorPlanning
BBa_K156023Promoter + RBS + phaC1 + double terminatorPlanning


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