Project

From 2012.igem.org

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Contents

Results

LovTAP

Results

LovTap 2.png

Plans

UVR8

Results

Uvrr.jpg


Plans

UV response screening with plate reader

FACS

UVR8 fusion purification: In vitro studies

Find constants for: Dimerization Monomerization DNA binding etc.

Join UVR8 and pABA systems/RecA and show increased E.coli UV-B tolerance (sun cream properties)

p-ABA generator

Results

agarose gel
Eth ladder paba.jpg
Ladder, A NheI/PstI, A XbaI/PstI, B NheI/PstI, B XbaI/PstI

As the promoter J23100 has two NheI restriction sites, construct A is cut twice (lane 2) while the promoterless construct B is cut only once (lane 4).

Plans

Cloning of B downstream of 1. the Tet promoter and 2. the PL dual input promoter BBa_K909011 containing the TetO1 and LacO1 operator sites.





References

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