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File:Band with CAR success 50ng.JPG
From 2012.igem.org
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This gel shows 2 bands of CAR that indicate successful mutagenesis of the first PstI site in CAR. The CAR gene is contained in the PET vector. Lane 1 contains the 1 kb plus ladder, while lanes 2,3 contained samples in which 5ng of original CAR+PET plasmid were used, lanes 4,5 contained samples in which 20ng of original plasmid were used, and lanes 6,7 contained 50ng of plasmid sample and underwent mutagenesis by PCR amplification successfully. The bands are slightly over the 9kb band of the ladder, at about 10kb, which is roughly the size of the 10,090bp plasmid.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 02:40, 22 August 2012 | | 1,017×661 (74 KB) | Tsarina.VanWieren (Talk | contribs) | (This gel shows 2 bands of CAR that indicate successful mutagenesis of the first PstI site in CAR. The CAR gene is contained in the PET vector. Lane 1 contains the 1 kb plus ladder, while lanes 2,3 contained samples in which 5ng of original CAR+PET plasmid) |
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